A MICROTUBULE-ASSOCIATED PROTEIN IN THE MITOTIC SPINDLE AND THE INTERPHASE NUCLEUS
A MICROTUBULE-ASSOCIATED PROTEIN IN THE MITOTIC SPINDLE AND THE INTERPHASE NUCLEUS
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DOI：
10.1038/295248a0
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发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
MCINTOSH, JR
中科院分区：
文献类型：
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作者：
IZANT, JG;WEATHERBEE, JA;MCINTOSH, JR
有丝分裂过程中染色体的准确分离取决于纺锤体微管的可控聚合和功能。在体外聚合循环中与微管共沉积的装配促进多肽是体内有丝分裂装置形成和功能调节的有吸引力的候选者。然而，以往的工作表明，哺乳动物大脑或组织培养细胞中的微管相关蛋白(MAP)的主要种类要么与有丝分裂纺锤体1无关，要么与有丝分裂微管和间期细胞质微管2-4相关。因此，我们寻求一种新的方法来识别纺锤体调节因子。在这里报道的研究中，已经制备了针对HeLa细胞的MAP组分的单抗。其中一个杂交瘤克隆产生了与分子量为∼200,000的多肽结合的免疫球蛋白。在细胞分裂期间，这种抗原与有丝分裂装置中的纤维相联系，但在间期，它主要位于细胞核中。令人惊讶的是，这种抗原也可以在循环中的人类红细胞膜上检测到。这个纺锤体成分不同于以前描述的图谱，可能代表着有丝分裂装置的组装和功能中的一个调节因子。
Accurate chromosome segregation during mitosis is contingent on the controlled polymerization and function of spindle microtubules. Assembly-promoting polypeptides that co-sediment with microtubules during cycles of polymerizationin vitroare attractive candidates for regulators of the formation and function of the mitotic apparatusin vivo. However, previous work has shown that the major species of microtubule-associated proteins (MAPs) from mammalian brain or tissue culture cells are either not associated with the mitotic spindle1or are associated with both mitotic and interphase cytoplasmic microtubules2–4. We have therefore sought a novel way to identify spindle regulatory factors. In the study reported here, monoclonal antibodies have been prepared against a MAP fraction from HeLa cells. One of the resulting hybridoma clones produces IgG that binds to a polypeptide of molecular weight ∼200,000. During cell division this antigen is associated with fibres in the mitotic apparatus, but in interphase it is located primarily in the nucleus. Surprisingly, the antigen is also detectable on the plasma membrane of circulating human erythrocytes. This spindle component is distinct from previously characterized MAPs and may represent a regulatory factor in the assembly and function of the mitotic apparatus.