Hematopoietic Stem and Progenitor Cell Identification and Transplantation in Zebrafish.
Hematopoietic Stem and Progenitor Cell Identification and Transplantation in Zebrafish.
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DOI：
10.1007/978-1-0716-2679-5_15
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发表时间：
2023-01-01
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影响因子：
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通讯作者：
Tamplin, Owen J
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文献类型：
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作者：
Fraint, Ellen;Lv, Peng;Tamplin, Owen J
斑马鱼作为一种模式生物，以其多功能遗传学、快速发育和简单的实时成像而闻名。由于其高度保守的个体发育和基因调控网络，它是研究造血作用的绝佳模型。最近开发的高度特异性转基因报告系可以对活体斑马鱼的造血干细胞和祖细胞（HSPC）进行直接成像和跟踪。这些报告系还可用于 HSPC 的荧光激活细胞分选 (FACS)。与哺乳动物模型类似，HSPC 可以通过移植来重建斑马鱼受体的整个造血系统。然而，斑马鱼为研究 HSPC 生物学提供了独特的优势，例如胚胎移植和高通量化学筛选。本章将概述在斑马鱼中识别、分离和移植 HSPC 所需的方法。
The zebrafish as a model organism is well known for its versatilegenetics, rapid development, and straightforward live imaging. It is an excellent model to study hematopoiesis because of its highly conserved ontogeny and gene regulatory networks. Recently developed highly specific transgenic reporter lines have allowed direct imaging and tracking of hematopoietic stem and progenitor cells (HSPCs) in live zebrafish. These reporter lines can also be used for fluorescence-activated cell sorting (FACS) of HSPCs. Similar to mammalian models, HSPCs can be transplanted to reconstitute the entire hematopoietic system of zebrafish recipients. However, the zebrafish provides unique advantages to study HSPC biology, such as transplants into embryos and high-throughput chemical screening. This chapter will outline the methods needed to identify, isolate, and transplant HSPCs in zebrafish.