Comparative assessment of variation among sorghum germplasm accessions using seed morphology and RAPD measurements
Comparative assessment of variation among sorghum germplasm accessions using seed morphology and RAPD measurements
复制标题
DOI：
10.2135/cropsci2002.0291
复制
发表时间：
2002-01-01
期刊：
影响因子：
2.3
通讯作者：
Mullet, JE
中科院分区：
文献类型：
--
作者：
Dahlberg, JA;Zhang, X;Mullet, JE
高粱种质资源目前有超过4.2万份，数量太大，无法有效管理。本研究的具体目的是比较选用高粱（sorghum bicolor(L.)）的农艺描述子与基于随机扩增多态性DNA （RAPD）指纹图谱的系统发育类群。Moench)比赛。我们的目的是确定一种使用农艺描述符的方法，该方法最接近RAPD标记产生的分组。94个高粱品种被划分为5个主要品种中的4个。采用不同的农艺性状聚类方法与RAPD标记聚类方法进行了比较。采用的四种聚类方法与RAPD指纹图谱提供的信息之间的相似性程度各不相同。检验2使用完整数据集通过z分数和聚类分析对数据进行标准化，为双色人种提供了最高的相似性得分；而检验3使用主成分分析中选取的简化变量集通过z分数和聚类分析对数据进行标准化，为几内亚人种提供了最高的相似性得分。试验1，随机选择，尾状核和硬脑膜最高。当对所有种族取平均值时，测试2提供了最高的相似性得分。本研究的结果表明，没有一种利用农艺描述符的方法能与RAPD标记产生的分类非常接近。这些结果强调需要进一步研究评估用于开发核心收藏品及其有效性的技术。
The sorghum germplasm collection currently contains over 42 000 accessions, a number that is too large to manage efficiently. The specific objective or this research was to compare clusters developed from agronomic descriptors with phylogenetic groupings based on random amplified polymorphic DNA (RAPD) fingerprinting of selected sorghum [Sorghum bicolor(L.) Moench] races. Our intent was to identify one approach using agronomic descriptors that would most closely approximate the groupings produced by RAPD markers. Ninety-four accessions of sorghum were grouped into four of the five major races. Differences among accessions determined by various clustering procedures based on agronomic characteristics were compared with clusters developed by means of RAPD markers. Each race varied in the degree of similarity between the four clustering approaches taken and the information provided by RAPD fingerprinting. Test 2, standardization of data by Z-scores and cluster analysis using the complete set of data, provided the highest Similarity score for the race bicolor, while Test 3, standardization of data by Z-scores and cluster analysis based on a reduced set of variables selected from principle component analysis, provided the highest similarity scores for the races guinea. Test 1, random selection, was highest for the races caudatum and durra. When averaged over all the races, Test 2 provided the highest similarity score. The results of this study indicate that no one approach to develop clusters by means of agronomic descriptors closely approximate the groupings produced by RAPD markers. These results underscore the need for further research in the evaluation of techniques used to develop core collections and their validity.