Detection of canine brucellosis by a rapid agglutination test using Rhizobium tropici as antigen.
Detection of canine brucellosis by a rapid agglutination test using Rhizobium tropici as antigen.
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使用热带根瘤菌作为抗原，通过快速凝集试验检测犬布鲁氏菌病。
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发表时间：
2010
期刊：
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通讯作者：
M. Zorlutuna
中科院分区：
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作者：
U. S. Ucan;Z. Aras;M. Zorlutuna
摘要犬种布鲁氏菌感染可引起犬和人的各种生殖功能障碍。由于人畜共患病的风险，本研究的目的是评估在血清学方法中使用相关细菌的抗原(如热带根瘤菌)来证明犬循环中的抗布鲁氏菌抗体的可行性。为此，用犬布鲁氏菌NCTC 10854株抗原和非特异性快速玻片凝集试验(RSAT)，用改良微量平板凝集试验、2-巯基乙醇试管凝集试验(2Me-TAT)和间接酶联免疫吸附试验(I-EL ISA)，对13 5只犬的布氏杆菌病血清阳性率进行了检测。I-ELISA法的犬布鲁氏菌病患病率为21.5%，MPAT法(25.2%)和2Met-TAT法(22.2%)稍高，两种方法的相对性能仍高于I-EL ISA法(一致性、敏感性和特异度均在90%以上)。来自英镑和雌性的狗被感染的程度明显最高。与I-ELISA相比，RSAT检测的假阳性和假阴性血清比例显著升高(分别为22.6%和69.0%)，导致其相对效价较低(一致性：67.4%，敏感性：31.0%，特异性：77.4%)。犬巴氏杆菌与热带巴氏杆菌的遗传相似性为30.8%。这些结果表明，犬布鲁氏菌病是中央安纳托利亚地区比较常见的感染，以热带布鲁氏菌为抗原的RSAT不能用于该病的血清学诊断。
SUMMARY The Brucella canis infection induces various reproductive disorders in dogs and also in humans. The aim of this study was to evaluate the feasability to use antigens from related germs, such as Rhizobium tropici in serological methods for evidencing circulating anti-Brucella antibodies in dogs, because of the zoonotic risk. For that, brucellosis seroprevalence on 135 dogs stemming from pounds or from the Faculty of Veterinary Medicine of the Konya region was determined with specific serological tests (Modified Micro Plate Agglutination Test (MPAT), 2-Mercaptoethanol Tube Agglutination Test (2Me-TAT) an indirect ELISA (I-ELISA)) using B. canis NCTC 10854 strain antigens and with a non specific rapid slide agglutination test (RSAT) using R. tropici CIAT 899 strain as antigens. The canine brucellosis prevalence was 21.5% with I-ELISA and was weakly higher with the MPAT (25.2%) and the 2ME-TAT (22.2%), the relative performances of these 2 specific tests compared to I-ELISA remaining elevated (Agreements, sensibility and specificity around 90% or more). Dogs from pounds and females were significantly the most infected. By contrast, the proportions of false positive and false negative sera with RSAT were markedly elevated (22.6% and 69.0% respectively) leading to low relative performances compared to the I-ELISA (agreement: 67.4%, sensibility: 31.0% and specificity: 77.4%). Genetic similarity between B. canis and R. tropici was determined 30.8% by RAPD-PCR. These results demonstrated that canine brucellosis is a relatively common infection in the Central Anatolia and that the RSAT using R. tropici as antigens cannot be use for the serological diagnosis of the disease.