Quantification of small molecule-receptor affinities and kinetics by acoustic profiling.
Quantification of small molecule-receptor affinities and kinetics by acoustic profiling.
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通过声学分析量化小分子受体亲和力和动力学。
DOI：
10.1089/adt.2006.4.565
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发表时间：
2006
影响因子：
1.8
通讯作者：
Cooper,MatthewA
中科院分区：
文献类型：
--
作者：
Li,Xin;Thompson,KevinSJ;Godber,Ben;Cooper,MatthewA
利用 Akubio（英国剑桥）的共振声学分析 (RAP™) 的无标记 RAP®id4™ 系统用于确定几种不同小分子-受体相互作用的亲和力和动力学。这是通过各种特定的耦合化学将目标受体附着到石英晶体谐振器的表面，然后通过基于微流体的输送系统将小分子量配体应用到受体上来实现的。确定非常弱的相互作用的结合顺序，例如辅因子与葡萄糖脱氢酶的结合。可以确定生物素与特定抗体的结合以及几种低分子量磺酰胺类似物与人碳酸酐酶亚型 II 的结合的中等相互作用亲和力和结合动力学。平衡结合常数与通过数据动力学分析获得的值以及之前发表的使用表面等离子体共振、停流荧光和等温滴定量热法获得的值基本一致。
The label-free RAP♦id4™ system that exploits resonant acoustic profiling (RAP™) from Akubio (Cambridge, UK) was used to determine the affinity and kinetics for several different small molecule–receptor interactions. This was achieved by attaching the target receptor to the surface of quartz crystal resonators through a variety of specific coupling chemistries, followed by application of a small-molecular-weight ligand to the receptor via a microfluidic flow-based delivery system. Rank order of binding was determined for very weak interactions such as cofactor binding to glucose dehydrogenase. Moderate interaction affinities and binding kinetics could be determined for biotin binding to a specific antibody, and also for several low-molecular-weight sulfonamide analogues binding to human carbonic anhydrase isoform II. The equilibrium binding constants were in general agreement with the values obtained by kinetic analysis of the data, as well as with previously published values obtained using surface plasmon resonance, stopped flow fluorescence, and isothermal titration calorimetry.
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对牛碳酸酐酶 II 具有强亲和力的新型苯磺酰胺的合成及其构效关系。
影响因子：
2.7
作者：
S. Poulsen;L. Bornaghi;P. Healy
通讯作者：
P. Healy
通过声剪切波传播检测表面附着的钙调蛋白的构象化学。
影响因子：
--
作者：
Xiaomeng Wang;J. S. Ellis;E. Lyle;Priyanka Sundaram;M. Thompson
通讯作者：
M. Thompson
液体中厚度剪切模式声波传感器的质量响应作为中心误导法则
DOI：
10.1109/freq.1997.638529
发表时间：
1997
期刊：
Proceedings of International Frequency Control Symposium
影响因子：
--
作者：
M. Thompson;G. Hayward
通讯作者：
G. Hayward
影响因子：
12.6
作者：
Marx, KA;Zhou, TA;Braunhut, SJ
通讯作者：
Braunhut, SJ