Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster.
Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster.
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作者：
Nava Gonzales C;McKaughan Q;Bushong EA;Cauwenberghs K;Ng R;Madany M;Ellisman MH;Su CY
感觉神经元的生物物理特性受其形态测量和形态特征的影响，其精确测量需要高质量的体积电子显微镜（EM）。然而，系统的调查，确定神经元的纳米级特征是稀缺的。在这里，我们描述了果蝇嗅觉受体神经元（ORN）在大多数遗传识别的感觉毛的形态。通过分析冷冻固定的触角组织的连续块面电子显微镜图像，我们编译了一个广泛的形态测量数据集的基础上重建的3D模型的40个确定的触角ORN类型的33。此外，我们观察到多个新的功能，包括细胞外空泡内的感觉腔，复杂的树突状分支，线粒体富集在选择的ORN，新的感觉器类型，空感器不含神经元，这提出了新的问题有关的细胞生物学和感觉神经生物学。我们的系统调查是至关重要的，为未来的调查如何感觉神经元的大小和形状影响他们的反应，灵敏度和电路功能。
The biophysical properties of sensory neurons are influenced by their morphometric and morphological features, whose precise measurements require high-quality volume electron microscopy (EM). However, systematic surveys of nanoscale characteristics for identified neurons are scarce. Here, we characterize the morphology of Drosophila olfactory receptor neurons (ORNs) across the majority of genetically identified sensory hairs. By analyzing serial block-face electron microscopy images of cryofixed antennal tissues, we compile an extensive morphometric data set based on 122 reconstructed 3D models for 33 of the 40 identified antennal ORN types. Additionally, we observe multiple novel features—including extracellular vacuoles within sensillum lumen, intricate dendritic branching, mitochondria enrichment in select ORNs, novel sensillum types, and empty sensilla containing no neurons—which raise new questions pertinent to cell biology and sensory neurobiology. Our systematic survey is critical for future investigations into how the size and shape of sensory neurons influence their responses, sensitivity, and circuit function.