Dynamic changes in the gene expression of zebrafish Reelin receptors during embryogenesis and hatching period
Dynamic changes in the gene expression of zebrafish Reelin receptors during embryogenesis and hatching period
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DOI：
10.1111/j.1440-169x.2012.01327.x
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发表时间：
2012-02-01
影响因子：
2.5
通讯作者：
Katsuyama, Yu
中科院分区：
文献类型：
--
作者：
Imai, Hideaki;Oomiya, Yoshihiro;Katsuyama, Yu
脊椎动物的脑形态表现出巨大的进化多样性，但脊椎动物大脑独特的共同形态特征之一是神经元的层状组织。由于Reelin信号在哺乳动物大脑的层状结构发育中起着重要作用，对低等脊椎动物的Reelin信号的研究将为脊椎动物脑形态发生的进化提供一些启示。虽然已经报道了斑马鱼Reelin的配体和Dab1的同源信号通路，但斑马鱼的Reelin受体分子尚未见报道。在这里，我们寻找了斑马鱼受体同源物vldlr和ApoER2的cDNA序列，并通过原位杂交检测了它们的表达模式。比较了reelin、dab1、vldlr和ApoER2基因在斑马鱼中枢神经系统中的发育基因表达模式，发现它们在发育中的顶盖、小脑等层状结构以及大脑皮层等非层状结构中均有显著表达。Reelin受体表现出不同的空间和时间基因表达。这些结果表明，Reelin受体的复制和随后的功能多样性可能有助于脊椎动物大脑的形态和功能进化。
The brain morphology of vertebrates exhibits huge evolutionary diversity, but one of the shared morphological features unique to vertebrate brain is laminar organization of neurons. Because the Reelin signal plays important roles in the development of the laminar structures in mammalian brain, investigation of Reelin signal in lower vertebrates will give some insights into evolution of vertebrate brain morphogenesis. Although zebrafish homologues of Reelin, the ligand, and Dab1, a cytoplasmic component of the signaling pathway, have been reported, the Reelin receptor molecules of zebrafish are not reported yet. Here, we sought cDNA sequence of zebrafish homologue of the receptors, vldlr and apoer2, and examined their expression patterns by in situ hybridization. Developmental gene expression pattern of reelin, dab1, vldlr, and apoer2 in the central nervous system of zebrafish was compared, and their remarkable expression was detected in the developing laminar structures, such as the tectum and the cerebellum, and also non-laminated structures, such as the pallium. The Reelin receptors exhibited different spatial and temporal gene expression. These results suggest a possibility that duplication and subsequent functional diversity of Reelin receptors contributed to the morphological and functional evolution of vertebrate brain.