Tissue collection methods for antler research
Tissue collection methods for antler research
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DOI：
10.1076/ejom.41.1.23.28106
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发表时间：
2003-02-01
期刊：
影响因子：
--
通讯作者：
Suttie, J. M.
中科院分区：
文献类型：
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作者：
Li, C.;Suttie, J. M.
鹿角的快速生长使其成为研究组织再生的潜在优秀模型。为了促进这一点，我们通过多年的鹿角研究，开发和完善了鹿角组织采样方法。本研究将鹿角组织分为鹿干组织、鹿胚组织和鹿角生长中心三大类。对于茎组织取样，在划定边界后，可以使用一对大鼠齿钳很容易地从底层骨上剥下整个初始致膝骨膜（直径约22毫米）。椎弓根和鹿角的根尖和末梢骨膜/软骨膜只有分别切成4象限和0.5 cm宽的条状才能完整剥离。鹿角胚皮包括本身胚皮、增强骨膜和休眠骨膜。使用一次性切片机刀片将囊胚本身分为4个象限后采样。采用与椎弓根和鹿角周围骨膜取样相同的方法采集增强骨膜和休眠骨膜。根据不同的形态学标记，用手术刀将鹿角生长中心分为5层。利用酶解法可进一步将表皮层分离为真皮层和表皮层，以研究组织间的相互作用。我们相信，现代技术的应用与本文报道的组织收集方法相结合，将极大地促进这些有价值模型的建立。
The rapid growth of deer antlers makes them potentially excellent models for studying tissue regeneration. In order to facilitate this, we have developed and refined antler tissue sampling methods through years of antler research. In the study, antler tissues were divided into three main groups: antler stem tissue, antler blastema and antler growth centre. For sampling stem tissue, entire initial antlerogenic periosteum (around 22 mm in diameter) could be readily peeled off from the underlying bone using a pair of rat-toothed forceps after delineating the boundary. Apical and peripheral periosteum/perichondrium of pedicle and antler could only be peeled off intact when they were cut into 4 quadrants and 0.5 cm-wide strips respectively. Antler blastema included blastema per se, and potentiated and dormant periostea. Blastema per se was sampled after it was divided into 4 quadrants using a disposable microtome blade. Potentiated and dormant periostea were collected following the same method used for sampling peripheral periosteum of pedicle and antler. The antler growth centre was divided with a scalpel into 5 layers according to distinctive morphological markers. The apical skin layer could be further separated into dermis and epidermis using enzyme digestion for the study of tissue interaction. We believe that the application of modern techniques coupled with the tissue collection methods reported here will greatly facilitate the establishment of these valuable models.