Elastase-producing Pseudomonas aeruginosa degrade plasma proteins and extracellular products of human skin and fibroblasts, and inhibit fibroblast growth
Elastase-producing Pseudomonas aeruginosa degrade plasma proteins and extracellular products of human skin and fibroblasts, and inhibit fibroblast growth
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DOI：
10.1016/s0882-4010(02)00197-3
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发表时间：
2003-01-01
影响因子：
3.8
通讯作者：
Björck, L
中科院分区：
文献类型：
--
作者：
Schmidtchen, A;Holst, E;Björck, L
静脉源性腿部溃疡是一种影响0.1-0.2%人群的疾病。众所周知，几乎所有慢性溃疡都由不同的细菌定植，如葡萄球菌、肠球菌和铜绿假单胞菌。我们在这里报告，铜绿假单胞菌，表达主要的金属蛋白酶弹性蛋白酶，诱导补体C3，各种抗蛋白酶，激肽原，成纤维细胞蛋白，蛋白多糖（PG）在体外降解，从而模仿蛋白水解活性先前确定在慢性溃疡液在体内。产生弹性蛋白酶的铜绿假单胞菌分离株显示出显著降解人伤口液以及离体人皮肤蛋白。含有弹性蛋白酶的铜绿假单胞菌条件培养基和纯化的弹性蛋白酶抑制成纤维细胞生长。这些影响，结合发现，在离体伤口液中检测到蛋白酶的产生，表明细菌蛋白酶在慢性溃疡中起致病作用。(C)2003爱思唯尔科技有限公司版权所有。
Leg ulcers of venous origin represent a disease affecting 0.1-0.2% of the population. It is known that almost all chronic ulcers are colonized by different bacteria, such as staphylococci, enterococci and Pseudomonas aeruginosa. We here report that P. aeruginosa, expressing the major metalloproteinase elastase, induces degradation of complement C3, various antiproteinases, kininogens, fibroblast proteins, and proteoglycans (PG) in vitro, thus mimicking proteolytic activity previously identified in chronic ulcer fluid in vivo. Elastase-producing P. aeruginosa isolates were shown to significantly degrade human wound fluid as well as human skin proteins ex vivo. Elastase-containing conditioned P. aeruginosa medium and purified elastase inhibited fibroblast cell growth. These effects, in conjunction with the finding that proteinase production was detected in wound fluid ex vivo, suggest that bacterial proteinases play a pathogenic role in chronic ulcers. (C) 2003 Elsevier Science Ltd. All rights reserved.