SARS-CoV-2 detection in formalin-fixed paraffin-embedded tissue specimens from surgical resection of tongue squamous cell carcinoma
SARS-CoV-2 detection in formalin-fixed paraffin-embedded tissue specimens from surgical resection of tongue squamous cell carcinoma
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DOI：
10.1136/jclinpath-2020-206635
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发表时间：
2020-11-01
影响因子：
3.4
通讯作者：
Barberis, Massimo
中科院分区：
文献类型：
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作者：
Guerini-Rocco, Elena;Taormina, Sergio Vincenzo;Barberis, Massimo
在严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）大流行中，病理学家可能会接触到处理手术标本的感染。与实验室安全程序有关的准则已经发布。然而，缺乏对活检和手术切除标本的研究。在这里，我们报告了在术后发生COVID-19的患者的舌鳞状细胞癌手术切除的福尔马林固定石蜡包埋样本中检测到SARS-CoV-2。采用实时荧光定量PCR技术检测SARS-CoV-2株RNA在肿瘤和正常颌下腺组织中的表达。转移淋巴结和反应性淋巴结中未发现病毒RNA。我们证明，即使在COVID-19疾病发展之前，SARS-CoV-2 RNA也可以在常规组织病理学样本中检测到。这些发现可以提供关于感染或病毒储存库的可能部位的重要信息，并强调在处理样本之前正确处理和固定的必要性。
In the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, pathologists can be exposed to infection handling surgical specimens. Guidelines related to safety procedures in the laboratory have been released. However, there is a lack of studies performed on biopsy and surgical resection specimens. Here we report the detection of SARS-CoV-2 in formalin-fixed paraffin-embedded samples from surgical resection of tongue squamous cell carcinoma of a patient who developed COVID-19 postsurgery. RNA of SARS-CoV-2 strain was detected in the tumour and the normal submandibular gland samples using real-time PCR-based assay. No viral RNA was found in metastatic and reactive lymph nodes. We demonstrated that SARS-CoV-2 RNA can be detected in routine histopathological samples even before COVID-19 disease development. These findings may give important information on the possible sites of infection or virus reservoir, and highlight the necessity of proper handling and fixation before sample processing.