Dietary fish oil and curcumin combine to modulate colonic cytokinetics and gene expression in dextran sodium sulphate-treated mice.
Dietary fish oil and curcumin combine to modulate colonic cytokinetics and gene expression in dextran sodium sulphate-treated mice.
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DOI：
10.1017/s0007114511000390
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发表时间：
2011-08
影响因子：
3.6
通讯作者：
Chapkin, Robert S.
中科院分区：
文献类型：
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作者：
Jia, Qian;Ivanov, Ivan;Zlatev, Zlatomir Z.;Alaniz, Robert C.;Weeks, Brad R.;Callaway, Evelyn S.;Goldsby, Jennifer S.;Davidson, Laurie A.;Fan, Yang-Yi;Zhou, Lan;Lupton, Joanne R.;McMurray, David N.;Chapkin, Robert S.
鱼油（FO）和姜黄素都具有抗肿瘤和抗炎的潜力。为了进一步探索它们对葡聚糖硫酸钠（DSS）诱导的结肠炎的联合作用，将C57 BL/6小鼠随机分配至四种脂肪酸含量不同的饮食（2 × 2设计），有或没有姜黄素补充（FO、FO +2%姜黄素、玉米油（对照，MO）或MO +2%姜黄素）。将小鼠暴露于饮用水中的DSS的一个或两个周期以分别诱导急性或慢性肠道炎症。暴露于单周期DSS治疗的FO喂养小鼠表现出最高的死亡率（40%，43只中的17只），而MO的死亡率最低（3%，29只中的1只）（P = 0.0008）。与对照组相比，在MO中添加姜黄素使死亡率增加（P = 0.003）至37%。与动物存活数据一致，在一个或两个周期的DSS治疗后，与MO相比，饮食FO和姜黄素均促进粘膜损伤/溃疡形成。相比之下，与其他饮食相比，FO和姜黄素联合喂养增强了慢性炎症的消退，并抑制了结肠粘膜中的关键炎症介质NF-κB（P <0.05）。粘液质微阵列分析显示，饮食FO，姜黄素和FO加姜黄素组合差异调制DSS治疗诱导的基因表达。这些结果表明，膳食脂质和姜黄素相互作用，以调节粘膜的稳态和慢性炎症的决议在结肠。
Both fish oil (FO) and curcumin have potential as anti-tumour and anti-inflammatory agents. To further explore their combined effects on dextran sodium sulphate (DSS)-induced colitis, C57BL/6 mice were randomised to four diets (2 × 2 design) differing in fatty acid content with or without curcumin supplementation (FO, FO + 2 % curcumin, maize oil (control, MO) or MO + 2 % curcumin). Mice were exposed to one or two cycles of DSS in the drinking-water to induce either acute or chronic intestinal inflammation, respectively. FO-fed mice exposed to the single-cycle DSS treatment exhibited the highest mortality (40 %, seventeen of forty-three) compared with MO with the lowest mortality (3 %, one of twenty-nine) (P = 0·0008). Addition of curcumin to MO increased (P = 0·003) mortality to 37 % compared with the control. Consistent with animal survival data, following the one- or two-cycle DSS treatment, both dietary FO and curcumin promoted mucosal injury/ulceration compared with MO. In contrast, compared with other diets, combined FO and curcumin feeding enhanced the resolution of chronic inflammation and suppressed (P < 0·05) a key inflammatory mediator, NF-κB, in the colon mucosa. Mucosal microarray analysis revealed that dietary FO, curcumin and FO plus curcumin combination differentially modulated the expression of genes induced by DSS treatment. These results suggest that dietary lipids and curcumin interact to regulate mucosal homeostasis and the resolution of chronic inflammation in the colon.