Determination of the ratio between mercaptalbumin and nonmercaptalbumin by HPLC with fluorescence probe specifically binding to albumin
Determination of the ratio between mercaptalbumin and nonmercaptalbumin by HPLC with fluorescence probe specifically binding to albumin
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特异性结合白蛋白的荧光探针高效液相色谱法测定硫醇白蛋白与非硫醇白蛋白的比例
DOI：
10.1016/j.jpba.2013.08.009
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发表时间：
2014
影响因子：
3.4
通讯作者：
Kuroda N.
中科院分区：
文献类型：
--
作者：
Ohyama K;Kishikawa N;Matsuo A;Imazato T;Ueki Y;Wada M;Nakashima K;Kuroda N.
以4-[4-（4-二甲氨基苯基）-5-苯基-1H-咪唑-2-基]苯甲酸甲酯（DAPIM）为荧光探针，建立了同时测定巯基白蛋白（HMA）和非巯基白蛋白（HNA 1）的高效液相色谱荧光（FL）法。HMA和HNA 1在离子交换柱上分离后，与DAPIM进行在线和柱后混合。DAPIM-白蛋白复合物产生荧光（λex 370 nm，λem 510 nm），而DAPIM溶液不产生荧光。方法用于健康志愿者和糖尿病患者血清中HMA和HNA 1的测定
A simple and selective HPLC-fluorescence (FL) method with FL probe, 4-[4-(4-dimethylaminophenyl)-5-phenyl-1H-imidazol-2-yl]benzoic acid methyl ester (DAPIM), for simultaneous determination of mercaptalbumin (HMA) and nonmercaptalbumin (HNA1) was developed. After HMA and HNA1 were separated on an ion-exchange column, they were on-line and post-column mixed with DAPIM. The DAPIM-albumin complex produces FL (λex 370 nm and λem 510 nm); however, DAPIM solution never gives the FL. Based on this mechanism, selective determination of HMA and HNA1 were achieved without any pretreatment and interfering peak. The proposed method was applied to the measurement of HMA and HNA1 in human serum of healthy volunteers and diabetes mellitus patients