Distinct regulatory control of the Brachyury gene in axial and non-axial mesoderm suggests separation of mesoderm lineages early in mouse gastrulation
Distinct regulatory control of the Brachyury gene in axial and non-axial mesoderm suggests separation of mesoderm lineages early in mouse gastrulation
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DOI：
10.1016/0925-4773(96)00520-5
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发表时间：
1996-05-01
影响因子：
2.6
通讯作者：
Stott, D
中科院分区：
文献类型：
--
作者：
Clements, D;Taylor, HC;Stott, D
短尾畸形是小鼠胚胎发育过程中前后轴正常延伸所必需的。包含相对于Brachyury转录起始的-500至+150的序列和报告基因lacZ的转基因概括了Brachyury表达的一些但不是全部元件。β-半乳糖苷酶表达见于6.5 d.p.c.但在结或脊索中没有可检测的报告基因表达。因此，在穿过原条的细胞中表达Brachyury所需的调控序列与在结中起始表达所需的调控序列不同。这表明，不同的或额外的信号参与激活的Brachyury在节点和脊索比那些诱导Brachyury在原始条纹。此外，数据表明轴向和非轴向中胚层与Brachyury表达的最早阶段不同的可能性。
Brachyury is required for the normal extension of the anteroposterior axis during mouse embryogenesis. A transgene comprising sequences from -500 to +150 relative to the start of Brachyury transcription, and the reporter gene lacZ, recapitulates some, but not all elements of Brachyury expression. beta-Galactosidase expression is seen in the primitive streak from 6.5 d.p.c. but there is no detectable reporter expression in the node or notochord. Thus, the regulatory sequences required for the expression of Brachyury in the cells traversing the primitive streak are distinct from those required for the initiation of expression in the node. This suggests that different or additional signals are involved in activation of Brachyury in the node and notochord than those inducing Brachyury in the primitive streak. Additionally, the data suggest the possibility that axial and non-axial mesoderm are distinct from the earliest stages of Brachyury expression.