Comparison of eleven commercially available rapid tests for detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis
Comparison of eleven commercially available rapid tests for detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis
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DOI：
10.1111/lam.12392
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发表时间：
2015-05-01
影响因子：
2.4
通讯作者：
Grunow, R.
中科院分区：
文献类型：
--
作者：
Zasada, A. A.;Forminska, K.;Grunow, R.
鼠疫耶尔森氏菌、炭疽杆菌和土拉弗朗西斯氏菌引起严重的人畜共患病，并有可能导致人类的高发病率和死亡率。在自然爆发和故意或意外释放这些病原体的情况下，快速检测细菌对于限制释放的负面影响至关重要。在本研究中，我们评估了11个市售的快速检测试剂盒，用于检测鼠疫耶尔森氏菌，炭疽B.和F.土拉菌的灵敏度，特异性和简单的程序。结果表明，用于检测高致病性细菌的快速且易于执行的侧流测定具有非常有限的灵敏度。相比之下，免疫渗滤试验显示出高灵敏度，但特异性有限，并且需要太复杂的程序，无法由非实验室工作人员（例如，消防、警察和紧急医疗人员等第一反应者）在现场应用。每一个样品--无论快速检测呈阴性还是阳性--都应该在参考实验室使用经过验证的方法进行重新检测。研究的意义和影响快速检测引起炭疽、鼠疫和兔热病的高致病性细菌对于限制潜在释放（自然、意外或故意）的负面影响至关重要。在这项研究中，商业上可获得的快速检测炭疽杆菌，鼠疫耶尔森氏菌和土拉弗朗西斯菌检测的灵敏度，特异性和易于执行方面进行了研究。研究表明了在测试和结果解释过程中可能面临的问题。这项研究的结论不仅有助于为特定的第一反应者群体选择最合适的检测方法，而且有助于在怀疑污染的情况下做出具有高度社会和经济影响的决定。快速检测引起炭疽、鼠疫和兔热病的高致病性细菌对于限制潜在释放的负面影响至关重要（自然、意外或故意）。在这项研究中，商业上可获得的快速检测炭疽杆菌，鼠疫耶尔森氏菌和土拉弗朗西斯菌检测的灵敏度，特异性和易于执行方面进行了研究。研究表明了在测试和结果解释过程中可能面临的问题。本研究的结论不仅有助于为特定的第一反应者群体选择最合适的检测方法，而且有助于在怀疑污染的情况下做出具有高度社会和经济影响的决策。
Yersinia pestis, Bacillus anthracis and Francisella tularensis cause serious zoonotic diseases and have the potential to cause high morbidity and mortality in humans. In case of natural outbreaks and deliberate or accidental release of these pathogens rapid detection of the bacteria is crucial for limitation of negative effects of the release. In the present study, we evaluated 11 commercially available rapid test kits for the detection of Y.pestis, B.anthracis and F.tularensis in terms of sensitivity, specificity and simplicity of the procedure. The results revealed that rapid and easy-to-perform lateral flow assays for detection of highly pathogenic bacteria have very limited sensitivity. In contrast, the immunofiltration assays showed high sensitivity but limited specificity and required a too complicated procedure to be applied in the field by nonlaboratory workers (e.g. First Responders like fire, police and emergency medical personnel). Each sample - whether tested negative or positive by the rapid tests - should be retested in a reference laboratory using validated methods.Significance and Impact of the StudyRapid detection of highly pathogenic bacteria causing anthrax, plague and tularemia is crucial for the limitation of negative effects of a potential release (natural, accidental or deliberate). In the study, commercially available rapid tests for detection of Bacillus anthracis, Yersinia pestis and Francisella tularensis were investigated in terms of sensitivity, specificity and ease-to-perform. The study showed problems which could be faced during testing and results interpretation. Conclusions from this study should be helpful not only in selection of the most appropriate test for particular group of First Responders but also in undertaking decisions in situation of a contamination suspicion which have high social and economical impacts.Significance and Impact of the Study: Rapid detection of highly pathogenic bacteria causing anthrax, plague and tularemia is crucial for the limitation of negative effects of a potential release (natural, accidental or deliberate). In the study, commercially available rapid tests for detection of Bacillus anthracis, Yersinia pestis and Francisella tularensis were investigated in terms of sensitivity, specificity and ease-to-perform. The study showed problems which could be faced during testing and results interpretation. Conclusions from this study should be helpful not only in selection of the most appropriate test for particular group of First Responders but also in undertaking decisions in situation of a contamination suspicion which have high social and economical impacts.