Physicochemical properties of detergent-solubilized photochemical reaction centers from two strains of Rhodopseudomonas spheroides.
Physicochemical properties of detergent-solubilized photochemical reaction centers from two strains of Rhodopseudomonas spheroides.
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两种球状红假单胞菌菌株的去污剂溶解的光化学反应中心的物理化学性质。
DOI：
10.1021/bi00554a020
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发表时间：
1980
期刊：
影响因子：
2.9
通讯作者：
M. le Maire
中科院分区：
文献类型：
--
作者：
E. Rivas;F. Reiss;M. le Maire
Emilio里瓦斯、* Frangoise Reiss-Husson和Marc le Maire 5摘要：通过在含有不同H2、SO含量的洗涤剂缓冲液中的沉降速度和沉降平衡分析，在洗涤剂溶液中表征从球形红单胞菌（野生型和R 26菌株）纯化的反应中心。在洗涤剂浓度范围内（每毫升0.5-2.0毫克十二烷基二甲胺氧化物），洗涤剂-反应中心复合物是单分散的;在H20-H2180缓冲液中的沉降平衡实验表明，R 26和野生型反应中心复合物的颗粒重量分别为138900和140700。在这些条件下，如通过使用14 C标记的去污剂的色谱法测定的，在R 26和野生型制剂中，每g蛋白质分别结合0.67和0.58 g的十二烷基二甲胺N-氧化物。近年来，人们对几种细菌，特别是球形红球藻（Rhodopropyleneisspheroides，R.）spheroides）。这些复合物由一个多链膜蛋白和一些相关分子组成，包括细菌叶绿素（BChl），细菌脱镁叶绿素（Bpheo），泛醌（UQ），铁，可能还有类胡萝卜素，是目前已知的能够在体外进行初级光化学反应的最简单的单元。虽然有详细的信息
Emilio Rivas,* Frangoise Reiss-Husson, and Marc le Maire5 abstract: Reaction centers purified from Rhodopseudo-monas spheroides (wild-type and R 26 strains) were charac-terized in detergent solutions by sedimentation velocity and sedimentation equilibrium analysis in detergent buffers con-taining various amounts of H2, sO. In therange of detergent concentrations studied (0.5-2.0 mg of dodecyldimethylamine TV-oxide per mL), the detergent-reaction center complexes are monodisperse; sedimentation equilibrium experiments in H20-H2180 buffers revealed particle weights of 138 900 and 140700 for the R 26 and wild-type reaction centercomplexes, respectively. Under these conditions 0.67 and 0.58 g of do-decyldimethylamine TV-oxide were bound per g of protein in R 26 and wild-type preparations, respectively, as determined by chromatography using 14C-labeled detergent. From theseRecently much progress has been made in the characterization of photochemical reaction centers isolated from several bacterial species, especially from Rhodopseudomonas spheroides (R. spheroides).* 1 These complexes, constituted by a multichain integralmembrane protein and a number of as-sociated molecules including bacteriochlorophyll (BChl), bacteriopheophytin (Bpheo), ubiquinone (UQ), iron, and possibly carotenoid, are the simplest units presently known which are capable of performingin vitro the primary photo-chemical reactions. Although detailed information exists