DIAGNOSIS OF HELICOBACTER-PYLORI INFECTION BY PCR - COMPARISON WITH OTHER INVASIVE TECHNIQUES AND DETECTION OF CAGA GENE IN GASTRIC BIOPSY SPECIMENS
DIAGNOSIS OF HELICOBACTER-PYLORI INFECTION BY PCR - COMPARISON WITH OTHER INVASIVE TECHNIQUES AND DETECTION OF CAGA GENE IN GASTRIC BIOPSY SPECIMENS
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DOI：
10.1128/jcm.33.10.2752-2756.1995
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发表时间：
1995-10-01
影响因子：
9.4
通讯作者：
GLUPCZYNSKI, Y
中科院分区：
文献类型：
--
作者：
LAGE, AP;GODFROID, E;GLUPCZYNSKI, Y
用特异性引物扩增ureC基因检测胃活检标本中幽门螺杆菌（本文称为ureC PCR）与其他常规侵入性方法进行比较通过对104例消化不良患者的组织切片进行细菌培养、快速尿素酶试验和Giemsa染色，尿素酶PCR、培养、快速尿素酶试验和姬姆萨染色阳性率分别为38.5%、36.5%、34.6%和33.7%。63例患者培养阴性，组织学检查阴性，快速尿素酶试验结果阴性，其中61例患者的ureC PCR也为阴性。ureC PCR在2例培养阴性患者中检出幽门螺杆菌。评估H. pylori基因直接来自胃活检样品和来自相应的H. pylori分离株，对31例患者的活检标本进行ureC和cagA靶向引物PCR。pylori分离株ureC PCR H阳性，cagA阳性的pylori菌株在同一患者的活检标本中也能产生阳性的cagA PCR片段，所有ureC PCR阳性的患者均进行了检测; 10/11例活检标本（91.7%）十二指肠溃疡患者携带H，pylori cagA阳性菌株，而26例慢性胃炎患者中19例cagA阳性，阳性率为73%。这些结果表明：1. ureC PCR至少与培养一样敏感，2. pylori cagA基因也可直接在活检标本中检测。
A PCR assay for the detection of Helicobacter pylori in gastric biopsy specimens with specific primers for ureC gene amplification (herein referred to as ureC PCR) was compared with other routine invasive methods (culture, the rapid-urease test, and Giemsa staining of histological sections),vith samples from a group of 104 consecutive dyspeptic patients, Bacteria were found in 40 (38.5%), 38 (36.5%), 36 (34.6%), and 35 (33.7%) of the patients by ureC PCR, culture, the rapid-urease test, and Giemsa stain, respectively. Sixty-three patients had negative cultures, negative histological examinations, and negative rapid-urease test results, and 61 of these patients were also negative by ureC PCR ureC PCR detected H, pylori in two culture-negative patients, In parallel, a PCR-based assay to detect the H, pylori cytotoxin-associated antigen (cagA) gene, a putative virulence gene, was also developed. To assess the likelihood of detection of H. pylori genes directly from gastric biopsy samples and from the corresponding H. pylori isolates, specimens from 31 patients were subjected to PCR with ureC- and cagA-targeting primers, All 31 biopsy specimens and the corresponding H. pylori isolates were positive in the ureC PCR H,pylori strains that were cagA positive also gave positive cagA PCR fragments with biopsy specimens from the same patients, All ureC PCR-positive patients were examined; biopsy specimens from 10 of 11 (91.7%) duodenal ulcer patients harbored H, pylori cagA-positive strains, whereas 19 of 26 (73%) of those from patients with chronic gastritis only were found to be cagA positive, These findings indicate first that ureC PCR is at least as sensitive as culture for diagnosing H, pylori infection and second that the presence of the H. pylori cagA gene can also be detected directly in biopsy specimens by PCR amplification.