ULTRASTRUCTURAL-LOCALIZATION OF RAT CLARA CELL 10 KD SECRETORY PROTEIN BY THE IMMUNOGOLD TECHNIQUE USING POLYCLONAL AND MONOCLONAL-ANTIBODIES
ULTRASTRUCTURAL-LOCALIZATION OF RAT CLARA CELL 10 KD SECRETORY PROTEIN BY THE IMMUNOGOLD TECHNIQUE USING POLYCLONAL AND MONOCLONAL-ANTIBODIES
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DOI：
10.1177/35.7.2438324
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发表时间：
1987-07-01
影响因子：
3.2
通讯作者：
WONGCHONG, ML
中科院分区：
文献类型：
--
作者：
BEDETTI, CD;SINGH, J;WONGCHONG, ML
使用单克隆抗体和亲和纯化的多克隆抗血清，针对从大鼠肺灌洗液中分离出的 10 KD 蛋白，我们通过后包埋蛋白 A-gold 技术将该蛋白定位在 Clara 细胞内。金颗粒位于大鼠克拉拉细胞的分泌颗粒上。当一抗预先被纯化的 10 KD 蛋白吸收时，超微结构免疫定位被废除。其他肺细胞，包括 II 型肺细胞和纤毛细胞，该技术呈阴性。这些结果证明了 Clara 细胞的分泌颗粒中存在 10 KD 蛋白，并支持该蛋白构成 Clara 细胞特异且独特的分泌产物的概念。
Using a monoclonal antibody and affinity-purified polyclonal antiserum against a 10 KD protein isolated from rat pulmonary lavage, we have localized the protein within Clara cells by a post-embedment protein A-gold technique. The gold particles were localized over the secretory granules of rat Clara cells. Ultrastructure immunolocalization was abolished when the primary antibodies were previously absorbed with purified 10 KD protein. Other pulmonary cells, including type II pneumocytes and ciliated cells, were negative with this technique. These results demonstrate the presence of the 10 KD protein in the secretory granules of the Clara cell and support the concept that this protein constitutes a specific and unique secretory product of Clara cells.