Large-scale mobilization and isolation of CD34+ cells from normal donors.
Large-scale mobilization and isolation of CD34+ cells from normal donors.
复制标题
从正常供体中大规模动员和分离 CD34 细胞。
DOI：
10.1038/sj.bmt.1702720
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Abonour,R
中科院分区：
文献类型：
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作者：
Croop,JM;Cooper,R;Seshadri,R;Fernandez,C;Graves,V;Kreissman,S;Smith,FO;Cornetta,K;Williams,DA;Abonour,R
我们描述了从志愿者、正常供体和异基因干细胞供体中收集和纯化外周血CD 34+细胞。使用外周静脉通路对68名志愿献血者进行了总计98次单采术。采集的有核细胞平均数为4.6× 1010，其中包括1.9× 108个CD 34+细胞，相当于2.7× 106个CD 34+细胞/kg。采集的CD 34+细胞数量在雄性和雌性之间没有差异，但与供体的体重和采集的有核细胞总数相关。使用Nexell Isolex 300 i细胞分离器从30个收集物中分离CD 34+细胞。平均回收总细胞的0.36%，包括43±18%的CD 34+细胞。CD 34+细胞占回收细胞的85±11%。回收的CD 34+细胞总数不受Isolex 300 i上有核细胞数量的影响。回收的CD 34+细胞百分比与置于Isolex 300 i上的CD 34+细胞数量无关。最终产物的纯度受CD 34+细胞数量的影响，但不受有核细胞总数的影响。对正常异基因干细胞供体进行另外38个CD 34+细胞分离，结果相似。这些观察结果进一步支持外周血CD 34+细胞收集和纯化的安全性和可行性。骨髓移植（2000）26，1271-1279。
We describe collection and purification of peripheral blood CD34+ cells from volunteer, normal donors and allogeneic stem cell donors. A total of 98 aphereses were performed on 68 volunteer donors using peripheral venous access. The mean number of nucleated cells collected was 4.6× 10 10 which included 1.9× 10 8 CD34+ cells corresponding to 2.7× 10 6 CD34+ cells/kg. The number of CD34+ cells collected did not differ between males and females but did correlate with the donor's weight and the total number of nucleated cells collected. The Nexell Isolex 300i cell separator was used to isolate CD34+ cells from 30 of the collections. A mean of 0.36% of the total cells was recovered and included 43±18% of the CD34+ cells. CD34+ cells represented 85±11% of the recovered cells. The total number of CD34+ cells recovered was not influenced by the number of nucleated cells placed on the Isolex 300i. The percentage of CD34+ cells recovered was not related to the number of CD34+ cells placed on the Isolex 300i. The purity of the final product was influenced by the number of CD34+ cells but not the total number of nucleated cells. An additional 38 CD34+ cell isolations were performed on normal allogeneic stem cell donors with similar results. These observations further support the safety and feasibility of peripheral blood CD34+ cell collection and purification. Bone Marrow Transplantation (2000) 26, 1271–1279.