Food odors trigger Drosophila males to deposit a pheromone that guides aggregation and female oviposition decisions
Food odors trigger Drosophila males to deposit a pheromone that guides aggregation and female oviposition decisions
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DOI：
10.7554/elife.08688
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发表时间：
2015-09-30
期刊：
影响因子：
7.7
通讯作者：
Potter, Christopher J.
中科院分区：
文献类型：
--
作者：
Lin, Chun-Chieh;Prokop-Prigge, Katharine A.;Potter, Christopher J.
动物利用嗅觉线索在复杂的环境中导航。食物的气味尤其能提供潜在觅食地点的重要信息。许多行为发生在食物部位，但食物气味如何调节这些部位的行为尚不清楚。我们以黑腹果蝇为动物模型，发现雄性果蝇在食物气味的苹果醋刺激下分泌信息素9-三醇。这种信息素是一种有效的聚集信息素，也是雌性产卵的引导信号。我们使用遗传、分子、电生理和行为方法来证明9-三糖可以激活触角碱性Or7a受体，这种受体可以被许多醇和醛激活，如绿叶挥发性e2 -己烯醛。我们证明Or7a阳性神经元或Or7a受体的缺失会破坏9-三糖和e2 -己烯醛的聚集行为和产卵位点选择。因此，9-三煤油通过Or7a起作用，将食物气味感知与聚集和产卵决定联系起来。
Animals use olfactory cues for navigating complex environments. Food odors in particular provide crucial information regarding potential foraging sites. Many behaviors occur at food sites, yet how food odors regulate such behaviors at these sites is unclear. Using Drosophila melanogaster as an animal model, we found that males deposit the pheromone 9-tricosene upon stimulation with the food-odor apple cider vinegar. This pheromone acts as a potent aggregation pheromone and as an oviposition guidance cue for females. We use genetic, molecular, electrophysiological, and behavioral approaches to show that 9-tricosene activates antennal basiconic Or7a receptors, a receptor activated by many alcohols and aldehydes such as the green leaf volatile E2-hexenal. We demonstrate that loss of Or7a positive neurons or the Or7a receptor abolishes aggregation behavior and oviposition site-selection towards 9-tricosene and E2-hexenal. 9-Tricosene thus functions via Or7a to link food-odor perception with aggregation and egg-laying decisions.