YAP mediates human decidualization of the uterine endometrial stromal cells
YAP mediates human decidualization of the uterine endometrial stromal cells
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DOI：
10.1016/j.placenta.2017.03.013
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发表时间：
2017-05-01
期刊：
影响因子：
3.8
通讯作者：
Huang, Wei
中科院分区：
文献类型：
--
作者：
Chen, Hengxi;Song, Yong;Huang, Wei
简介：子宫内膜间质细胞（ESCs）的蜕膜化是成功建立和维持妊娠的关键，涉及广泛的细胞增殖和分化。一种新建立的信号通路，Hippo/Yes相关蛋白（雅普）通路，在这些增殖过程中起着关键作用。我们先前的研究表明雅普在人胚胎干细胞中表达。然而，其在蜕膜化中的作用仍不清楚。本研究的目的是探讨雅普在人胚胎干细胞蜕膜化过程中的作用。方法：首先研究雅普在非妊娠妇女子宫内膜和妊娠妇女蜕膜中的表达。雅普的作用进行了研究，通过转染胚胎干细胞与mRNA沉默结构，并观察这种行动后，在体外诱导的蜕膜化的负面影响。结果：我们的研究结果表明，雅普的表达是较高的蜕膜细胞从早期妊娠蜕膜与胚胎干细胞从非妊娠子宫内膜。雅普和TEA结构域1（TEAD 1）的表达水平均增加在ESCs在体外蜕膜化和雅普在ESCs中的敲低引起的负作用后，在体外decidualizationin vitro.Discussion：我们的研究表明，雅普是上调的人蜕膜细胞相比，ESCs的蜕膜化。(C)2017爱思唯尔有限公司版权所有。
Introduction: The decidualization of uterine endometrial stromal cells (ESCs) is critical for the successful establishment and maintenance of pregnancy and involves extensive cell proliferation and differentiation. A newly established signaling pathway, the Hippo/Yes-associated protein (YAP) pathway, plays a critical role in these proliferation processes. Our previous study demonstrated that YAP is expressed in human ESCs. However, its role in decidualization remains unclear. The objective of the present study was to explore the role of YAP in the decidualization of human ESCs.Methods: The expression of YAP was first investigated in the endometrium of non-pregnant women and in the decidua of pregnant women. The role of YAP was investigated by transfecting ESCs with mRNA silencing constructs and observing the negative effects of this action upon decidualization induced in vitro.Results: Our results revealed that the expression of YAP was higher in decidual cells from early pregnant decidua compared with ESCs from non-pregnant endometrium. The expression levels of YAP and TEA domain 1 (TEAD1) were both increased in ESCs during in vitro decidualization and the knockdown of YAP in ESCs caused negative effects upon decidualization in vitro.Discussion: Our study suggests that YAP is upregulated in human decidual cells compared with ESCs and influences the decidualization of ESCs. (C) 2017 Elsevier Ltd. All rights reserved.