Cellular analysis of cutaneous leishmaniasis lymphadenopathy: Insights into the early phases of human disease
Cellular analysis of cutaneous leishmaniasis lymphadenopathy: Insights into the early phases of human disease
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DOI：
10.4269/ajtmh.2007.77.854
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发表时间：
2007-11-01
影响因子：
3.3
通讯作者：
Barral, Aldina
中科院分区：
文献类型：
--
作者：
Bomfim, Gloria;Andrade, Bruno B.;Barral, Aldina
淋巴结病是由维尼亚寄生虫引起的皮肤利什曼病 (CL) 的早期临床症状，可能有助于了解宿主对这些利什曼原虫的最初反应。我们报告了从患有淋巴结病但无溃疡（早期，N = 21）或淋巴结病和溃疡（晚期，N = 29）的皮肤利什曼病患者的淋巴结中获得的细胞特征。早期患者表现出较高比例的中性粒细胞、嗜酸性粒细胞和 CD8+ T 细胞。相反，CD19+ B 淋巴细胞和浆细胞在晚期患者中更常见。 IL-10 信号在晚期患者中显着较高；两组的 IFN-γ 或 IL-4 信号相似。这些数据强化了对初始混合 Th1-Th2 谱以及 CD8+ T 细胞在皮肤利什曼病中的早期作用的观察。此外，溃疡发展与 B 细胞增加之间存在时间顺序关系。 IL-10 在后期也会增加，对于限制组织损伤可能很重要。
Lymphadenopathy is an early clinical sign in cutaneous leishmaniasis (CL), caused by Viannia parasites, and may help to understand the initial host response to these species of Leishmania. We report on characteristics of cells obtained from lymph nodes from cutaneous leishmaniasis patients with lymphadenopathy without ulceration (early phase, N = 21) or lymphadenopathy and ulceration (late phase, N = 29). Early-phase patients exhibited a higher proportion of neutrophils, eosinophils, and CD8+ T cells. Conversely, CD19+ B lymphocytes and plasma cells were more frequently observed in late-phase patients. The signal for IL-10 was significantly higher in late-phase patients; signals for IFN-gamma or IL-4 were similar in both groups. These data reinforce observations of an initial mixed Th1-Th2 profile as well as the early role of the CD8+ T cell in cutaneous leishmaniasis. Additionally, there is a chronologic relationship between ulcer development and B-cell increase. IL-10 also increases at a late stage and may be important in limiting tissue damage.