The regulatory role of riboflavin in the drought tolerance of tobacco plants depends on ROS production
The regulatory role of riboflavin in the drought tolerance of tobacco plants depends on ROS production
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DOI：
10.1007/s10725-013-9858-8
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发表时间：
2014-04-01
影响因子：
4.2
通讯作者：
Zhang, Yaling
中科院分区：
文献类型：
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作者：
Deng, Benliang;Jin, Xuehui;Zhang, Yaling
核黄素（维生素B-2）是植物正常生长和发育所必需的。已有研究表明，核黄素的应用可以增强植物对病原菌的抗性。在这里，我们研究了核黄素在提高植物耐旱性（10%PEG 6000处理）中的作用。我们用5种不同水平的核黄素（0，4，20，100和500 μ M）处理4周龄的烟草植株5天，并检查它们的抗氧化反应和耐旱性水平。与对照相比，低和中等水平的核黄素处理增强了烟草植株的耐旱性，而较高浓度的核黄素（500 μ M）损害耐旱性。进一步的分析表明，500 μ M核黄素处理的植物积累了更高水平的ROS（O-2（-）和H2 O2）和脂质过氧化物比对照植物或低水平的核黄素处理的植物。与此观察结果相一致，抗氧化酶，如超氧化物歧化酶（SOD），过氧化氢酶（CAT），抗坏血酸过氧化物酶（APX）和谷胱甘肽还原酶（GR）的活性较高的植物处理低或中等（4，20和100 μ M）水平的核黄素与对照相比。我们还发现，叶绿素迅速降解的控制和500亩核黄素处理的植物在干旱胁迫条件下。此外，核黄素处理的植物的存活时间显着修改处理与还原型谷胱甘肽，一个众所周知的活性氧清除剂，在干旱胁迫条件下。因此，核黄素介导的ROS产生可能决定核黄素对烟草植物耐旱性的影响。
Riboflavin (vitamin B-2) is required for normal plant growth and development. Previous studies have shown that riboflavin application can enhance pathogen resistance in plants. Here, we investigated the role of riboflavin in increasing drought tolerance (10 % PEG6000 treatment) in plants. We treated 4 week-old tobacco plants with five different levels of riboflavin (0, 4, 20, 100 and 500 mu M) for 5 days and examined their antioxidant responses and levels of drought tolerance. Compared with the controls, low and moderate levels of riboflavin treatment enhanced drought tolerance in the tobacco plants, whereas higher concentrations of riboflavin (500 mu M) impaired drought tolerance. Further analysis revealed that plants treated with 500 mu M riboflavin accumulated higher levels of ROS (O-2 (-) and H2O2) and lipid peroxide than the control plants or plants treated with low levels of riboflavin. Consistent with this observation, the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) were higher in plants treated with low or moderate (4, 20 and 100 mu M) levels of riboflavin compared with the control. We also found that chlorophyll degraded rapidly in control and 500 mu M riboflavin-treated plants under drought stress conditions. In addition, the survival times of the riboflavin-treated plants were significantly modified by treatment with reduced glutathione, a well-known ROS scavenger, under drought stress conditions. Thus, riboflavin-mediated ROS production may determine the effects of riboflavin on drought tolerance in tobacco plants.