Analysis of 8-hydroxy-2′-deoxyguanosine in urine using high-performance liquid chromatography-electrospray tandem mass spectrometry
Analysis of 8-hydroxy-2′-deoxyguanosine in urine using high-performance liquid chromatography-electrospray tandem mass spectrometry
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DOI：
10.1016/j.jchromb.2007.03.016
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发表时间：
2007-06-15
影响因子：
3
通讯作者：
Peltonen, Kimmo
中科院分区：
文献类型：
--
作者：
Harri, Minna;Kasai, Hiroshi;Peltonen, Kimmo
8-羟基-2‘-脱氧鸟苷(8-OHdG)是一种广泛应用于DNA、蛋白质损伤和脂质过氧化相关的氧化应激的生物标志物。采用电喷雾电离(ESI)和使用同位素标记的8-OHdG作为内标，可以简单地定量尿样中的8-OHdG。高效液相色谱分离采用切峰技术和1.5 mm×120 mm分析阴离子交换柱。新方法只需要最少的样品处理，包括添加缓冲液和内标，然后在样品准备好进行分析之前进行离心。人尿样(n=246)中8-OHAG的含量范围为0。8-OHdG/g肌酐的正常值为0.49~14.27mUg/g肌酐。建立的高效液-质联用方法与现有的高效液相-电化学法具有良好的相关性，R-2值为0.8707。(C)2007 Elsevier B.V.保留所有权利。
8-Hydroxy-2'-deoxyguanosine (8-OHdG) is a widely used biomarker of oxidative stress in research related to DNA, protein damage as well as lipid peroxidation. HPLC-MS/MS with electrospray ionization (ESI) and the use of isotopically labelled 8-OHdG as an internal standard allows a simple quantification of 8-OHdG in urine samples. HPLC separation utilized the peak cutting technique and a 1.5 mm x 120 mm analytical anion exchange column. Novel method entails only minimal sample handling including the addition of a buffer and an internal standard followed by centrifugation before the samples are ready for analysis. The levels of 8-OHAG in human urine samples (n = 246) varied from 0. 16 to 16.48 mu g/L and the corresponding creatinine-normalized values were ranged from 0.49 to 14.27 mu g of 8-OHdG/g creatinine. The correlation between the developed HPLC-MS/MS method and the existing HPLC-EC method was good with an R-2 value of 0.8707. (c) 2007 Elsevier B.V. All rights reserved.