Increased cellular activity in rat insular cortex after water and salt ingestion induced by fluid depletion.
Increased cellular activity in rat insular cortex after water and salt ingestion induced by fluid depletion.
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DOI：
10.1152/ajpregu.00189.2002
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发表时间：
2003-04
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影响因子：
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通讯作者：
Cinthia V. Pastuskovas;M. Cassell;A. Johnson;R. Thunhorst
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作者：
Cinthia V. Pastuskovas;M. Cassell;A. Johnson;R. Thunhorst
岛叶皮层（IC）接收来自多个感觉系统的输入，包括味觉，以及来自监测身体电解质和液体平衡以及血压的受体。本研究分析了钠耗竭诱导的水钠摄入后IC细胞的代谢活性。给大鼠注射利尿剂呋塞米（10 mg/kg体重），5 min后注射血管紧张素转换酶抑制剂卡托普利（5 mg/kg体重）。90 min后，一些大鼠接受水和0.3 M NaCl饮用2 h，而另一些则没有。第三组可以获得水和盐水，但没有耗尽液体。所有大鼠均处死，处理脑组织进行Fos免疫反应（Fos-ir）。非耗尽的动物有弱到中等水平的Fos-IR内IC的分区。无液体通路的液体耗尽大鼠在IC的所有区域均显著增加Fos-ir。Fos-ir水平在饮用水和钠的液体耗尽大鼠中最高。Fos-ir水平在IC前部最高，在IC后部最低。这些结果暗示内脏，味道，和/或postingestional因素在IC细胞的代谢活性增加。
Insular cortex (IC) receives inputs from multiple sensory systems, including taste, and from receptors that monitor body electrolyte and fluid balance and blood pressure. This work analyzed metabolic activity of IC cells after water and sodium ingestion induced by sodium depletion. Rats were injected with the diuretic furosemide (10 mg/kg body wt), followed 5 min later by injections of the angiotensin-converting enzyme inhibitor captopril (5 mg/kg body wt). After 90 min, some rats received water and 0.3 M NaCl to drink for 2 h while others did not. A third group had access to water and saline but was not depleted of fluids. All rats were killed for processing of brain tissue for Fos-immunoreactivity (Fos-ir). Nondepleted animals had weak-to-moderate levels of Fos-ir within subregions of IC. Fluid-depleted rats without fluid access had significantly increased Fos-ir in all areas of IC. Levels of Fos-ir were highest in fluid-depleted rats that drank water and sodium. Fos-ir levels were highest in anterior regions of IC and lowest in posterior regions of IC. These results implicate visceral, taste, and/or postingestional factors in the increased metabolic activity of cells in IC.