DEVELOPMENT OF FIBROBLAST-SEEDED LIGAMENT ANALOGS FOR ACL RECONSTRUCTION
DEVELOPMENT OF FIBROBLAST-SEEDED LIGAMENT ANALOGS FOR ACL RECONSTRUCTION
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DOI：
10.1002/jbm.820291107
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发表时间：
1995-11-01
期刊：
影响因子：
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通讯作者：
ZAWADSKY, JP
中科院分区：
文献类型：
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作者：
DUNN, MG;LIESCH, JB;ZAWADSKY, JP
我们通过将关节内（前交叉韧带，ACL）或关节外（髌腱，PT）兔成纤维细胞接种到高强度可吸收胶原纤维支架上，在体外制造了“韧带模拟物”。成纤维细胞附着，增殖，并分泌新的胶原蛋白的韧带类似物在体外。成纤维细胞功能取决于组织培养基质（韧带类似物与组织培养板）和成纤维细胞来源（ACL与PT）。当在韧带类似物上培养时，PT成纤维细胞比ACL成纤维细胞增殖更快。ACL和PT成纤维细胞的胶原合成在韧带类似物上比在组织培养板上大约高10倍。胶原纤维支架的组成、结构和几何形状可以促进体外韧带类似物内的胶原合成。韧带类似物大致接近天然韧带组织的结构和强度。正在进行的体内研究表明，自体成纤维细胞种植的韧带类似物在植入膝关节后仍然具有活力。随着进一步的发展，韧带类似物可用作ACL重建手术的植入物。(C)John Wiley & Sons，Inc.
We fabricated ''ligament analogs'' in vitro by seeding high-strength resorbable collagen fiber scaffolds with intraarticular (anterior cruciate ligament, ACL) or extraarticular (patellar tendon, PT) rabbit fibroblasts. Fibroblasts attached, proliferated, and secreted new collagen on the ligament analogs in vitro. Fibroblast function depended on the tissue culture substrate (ligament analog vs. tissue culture plate) and the origin of the fibroblasts (ACL vs. PT). PT fibroblasts proliferated more rapidly than ACL fibroblasts when cultured on ligament analogs. Collagen synthesis by ACL and PT fibroblasts was approximately tenfold greater on ligament analogs than on tissue culture plates. The composition, structure, and geometry of the collagen fiber scaffolds may promote collagen synthesis within ligament analogs in vitro. Ligament analogs roughly approximate the structure and strength of native ligament tissue. Ongoing in vivo studies suggest that autogenous fibroblast-seeded ligament analogs remain viable after implantation into the knee joint. With further development, ligament analogs may be useful as implants for ACL reconstruction surgery. (C) 1995 John Wiley & Sons, Inc.