Fibroblast Activation Protein and Tertiary Lymphoid Structure in Colorectal Cancer Recurrence
Fibroblast Activation Protein and Tertiary Lymphoid Structure in Colorectal Cancer Recurrence
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DOI：
10.21873/anticanres.16099
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发表时间：
2022-12-01
影响因子：
2
通讯作者：
Eguchi, Hidetoshi
中科院分区：
文献类型：
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作者：
Hayase, Shimon;Miyoshi, Norikatsu;Eguchi, Hidetoshi
背景/目的：已知成纤维细胞激活蛋白 (FAP) 在结直肠癌 (CRC) 中具有预后意义。然而，FAP 和三级淋巴结构 (TLS) 在预测 CRC 复发的预后方面尚未相互关联。患者和方法：通过实时逆转录聚合酶链反应评估大阪国际癌症研究所 195 名 CRC 患者的 FAP 表达（第一个数据集）。然后对大阪大学医院 159 名 CRC 患者的浸润边缘 (IM) 和中央肿瘤 (CT) 进行免疫组织化学 (IHC) 染色（第二个数据集）。使用连续切片评估大阪大学医院 159 名 CRC 患者中 TLS 的存在情况。结果：与低 FAP 表达组 (n=83) 相比，高 FAP mRNA 表达组 (n=82) 的无复发生存率 (RFS) 较差 (p=0.004)。在第二个数据集中，与 CT 中 FAP 低表达且存在 TLS 的患者 (n=101) 相比，CT 中 FAP 高表达且不存在 TLS 的患者 (n=49) 的 RFS 显着较差 (p=0.002)。结论：CT 中的 FAP 结合 TLS 在预测根治性切除后的 CRC 复发方面具有显着的预后价值。
Background/Aim: Fibroblast activation protein (FAP) is known to have prognostic significance in colorectal cancer (CRC). However, FAP and tertiary lymphoid structures (TLSs) have not been associated with each other in predicting the prognosis of CRC recurrence. Patients and Methods: FAP expression was evaluated by real-time reverse transcription polymerase chain reaction in 195 CRC patients at Osaka International Cancer Institute (first data set). Immunohistochemistry (IHC) was then performed to stain FAP at the invasive margin (IM) and in the central tumour (CT) in 159 CRC patients at Osaka University Hospital (second data set). Consecutive slides were used to evaluate the presence of TLSs in 159 CRC patients from Osaka University Hospital. Results: The high FAP mRNA expression group (n=82) was associated with poor recurrence-free survival (RFS) compared with the low FAP expression group (n=83) (p=0.004). In the second data set, patients with high FAP expression in CT and TLS absence (n=49) showed significantly poorer RFS compared with those with low expression of FAP in CT and presence of TLSs (n=101) (p=0.002). Conclusion: FAP in the CT combined with TLSs was shown to have significant prognostic value in predicting CRC recurrence after curative resection.