Gene expression analysis of light-modulated germination in tomato seeds
Gene expression analysis of light-modulated germination in tomato seeds
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DOI：
10.1111/j.1469-8137.2009.02867.x
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发表时间：
2009-01-01
期刊：
影响因子：
9.4
通讯作者：
Francisco Botto, Javier
中科院分区：
文献类型：
--
作者：
Alejandra Auge, Gabriela;Perelman, Susana;Francisco Botto, Javier
P>番茄 (Solanum lycopersicum) 种子发芽可以通过远红光 (FRc) 的连续照射来抑制，并通过随后的红光脉冲重新诱导。在这项研究中，我们使用马铃薯 cDNA 微阵列，对经过 FRc 抑制处理（有或没有随后的红光脉冲）的种子进行了全局转录物分析。我们还鉴定并表征了参与光调制发芽的基因，作为光敏色素信号通路的元件。微阵列数据表明，FRc 对发芽的抑制涉及大量基因的诱导和显着较小数量的基因的抑制。多变量分析建立了依赖于生理处理和孵育时间的潜在表达模式，并鉴定了与休眠维持、抑制和促进发芽相关的不同基因组。我们发现 ELIP、CSN6、SOS2 和 RBP 与发芽的光控制有关。已知这些基因参与其他生理过程，但以前并未表明它们参与发芽。在光敏色素调节的发芽过程中，光质量通过改变某些基因组的表达来调节番茄种子转录组。另外，ELIP和GIGANTEA被证实为FRc抑制发芽过程中光敏色素A信号传导途径的组分。New Phytological (2009) 183:301-314doi:10.1111/j.1469-8137.2009.02867.x。
P>Tomato (Solanum lycopersicum) seed germination can be inhibited by continuous irradiation with far-red light (FRc) and re-induced by a subsequent red light pulse.In this study, we carried out a global transcript analysis of seeds subjected to FRc inhibitory treatment, with and without a subsequent red light pulse, using potato cDNA microarrays. We also identified and characterized genes involved in light-modulated germination as elements of the phytochrome signalling pathway.Microarray data showed that the inhibition of germination by FRc involves the induction of a large number of genes and the repression of a significantly smaller quantity. Multivariate analysis established an underlying pattern of expression dependent on physiological treatment and incubation time, and identified different groups of genes associated with dormancy maintenance, inhibition and promotion of germination. We showed that ELIP, CSN6, SOS2 and RBP are related to the photocontrol of germination. These genes are known to participate in other physiological processes, but their participation in germination has not been suggested previously.Light quality regulates the tomato seed transcriptome during phytochrome-modulated germination through changes in the expression of certain sets of genes. In addition, ELIP and GIGANTEA were confirmed as components of the phytochrome A signalling pathway during FRc inhibition of germination.New Phytologist (2009) 183: 301-314doi: 10.1111/j.1469-8137.2009.02867.x.