Synthesizing anode electrochemiluminescent self-catalyzed carbon dots-based nanocomposites and its application in sensitive ECL biosensor for microRNA detection
Synthesizing anode electrochemiluminescent self-catalyzed carbon dots-based nanocomposites and its application in sensitive ECL biosensor for microRNA detection
复制标题
阳极电化学发光自催化碳点纳米复合材料的合成及其在灵敏ECL生物传感器中microRNA检测的应用
DOI：
10.1016/j.snb.2019.127490
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Ruo Yuan
中科院分区：
文献类型：
--
作者：
Xia Zhong;Xue Li;Ying Zhuo;Yaqin Chai;Ruo Yuan
本工作通过将氨基修饰的碳点负载到AuNPs修饰的TiO2NPs(CDS-Au-PEI@Ti02)表面，制备了一种新型的自催化碳点电化学发光(ECL)发光体，并将其应用于检测miRNA-21的灵敏ECL生物传感器中。值得注意的是，所制备的CDS-Au-PEI@TiO2纳米复合材料表现出较强的初始电化学发光强度，加速了TiO2纳米粒子对共反应的H_2O_2的电化学还原，解决了传统的CDS-Au-PEI@TiO_2生物传感器中过硫酸盐强氧化的缺点，提高了检测灵敏度。此外，通过靶向诱导的催化发夹组件(CHA)扩增，可以在电极表面释放和捕获大量猝灭基团，进一步提高了检测灵敏度。结果表明，随着miRNA-21浓度从0.1fM增加到10fM，该生物传感器的电致发光响应逐渐降低，检测下限为0.03fM。本工作提供的策略为利用基于自催化碳点的ECL生物传感平台在实际细胞中检测miRNA开辟了新的途径。
In this work, a novel self-catalyzed carbon dots-based electrochemiluminescence (ECL) luminophore was prepared by loading the amino modified carbon dots onto the surface of AuNPs modified TiO2NPs (CDs-Au-PEI@TiO2) and applied in sensitive ECL biosensor for miRNA-21 detection. Significantly, the prepared CDs-Au-PEI@TiO2nanocomposites exhibited strong initial ECL intensity for the acceleration of TiO2NPs towards to the electrochemical reduction of co-reactive H2O2, addressing the shortcomings of the strong oxidizing nature of persulfate in traditional CDs-based ECL biosensings and enhancing the detection sensitivity. Moreover, by target-induced catalytic hairpin assembly (CHA) amplification, a large number of quenching groups could be released and captured on the electrode surface, further improving the detection sensitivity. As a consequence, the ECL response of the proposed biosensor gradually decreased as the concentration of miRNA-21 increasing from 0.1 fM to 10 pM with the detection limit of 0.03 fM. The strategy provided in this work opens up a new way for miRNA detection in actual cell using self-catalyzed carbon dots-based ECL biosensing platform.