Morphology and LPS content for the estimation of marine bacterioplankton biomass in the Ionian Sea
Morphology and LPS content for the estimation of marine bacterioplankton biomass in the Ionian Sea
复制标题
用于估算爱奥尼亚海海洋浮游细菌生物量的形态和 LPS 含量
DOI：
--
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
G. Maimone
中科院分区：
文献类型：
--
作者：
R. Ferla;A. Giudice;G. Maimone
The abundance, morphotypes and biomass of the bacterial assemblages were investigated in the Ionian Sea by using two different methods: the epifluorescent microscopy technique for enumerating and sizing bacterial cells, and the determination of bacterial lipopolysaccharides (LPS). Five bacterial morphotypes were distinguished: cocci, rods, coccobacilli, vibrios and spirillae. The proportions of cocci were higher than those of other morphotypes at every depth, ranging from 39% to 73%. Both rod-shaped bacteria and coccobacilli were homogenously distributed in the water column, while the proportions of vibrios were rather variable. Spirillae occurred only in surface samples and disappeared below 100 m. The two methodologies were compared: LPS concentrations showed a highly significant correlation with the bacterial numbers (P 3 respectively). LPS-derived cell carbon content on average was 23 fg C cell -1 , similar to the C amount derived by mean cell biovolume (19 fg C cell -1 ) and the biomass from two highly correlated methods (P -1 (Lee and Furhman, 1987) should be plausible for studying the biomass of the natural microbial populations in the study area. Nevertheless, the wide variability of the cell size classes, also along the whole water columns, questions the applicability of a constant conversion factor for all the marine ecosystems. Consequently, locally derived biomass estimates of bacteria are essential in order to obtain an accurate evaluation of the bacterial role in biogeochemical cycles.
