Glucocorticoid-regulated glycoprotein maturation in wild-type and mutant rat cell lines.
Glucocorticoid-regulated glycoprotein maturation in wild-type and mutant rat cell lines.
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DOI：
10.1083/jcb.103.6.2323
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发表时间：
1986-12
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影响因子：
--
通讯作者：
Yamamoto KR
中科院分区：
文献类型：
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作者：
Firestone GL;John NJ;Yamamoto KR
糖皮质激素可调节稳定感染的大鼠肝癌细胞系M1.54中小鼠乳腺肿瘤病毒（MTV）前体多聚蛋白的翻译后成熟。我们已经使用补体介导的细胞溶解来回收不能以酶调节的方式表达MTV细胞表面糖蛋白的M1.54变体（凡士通，G.L.，和K.R. Yamamoto，1983，Mol. Cell.生物学：3：149- 160）。一个这样的克隆分离物，CR 4，是类似于野生型的合成MTV的mRNA，生产的MTV糖蛋白前体（gPr 74 env）和糖基化的成熟产物（gp 51），和两个MTV磷蛋白的酶诱导的加工。相比之下，三种病毒细胞表面糖蛋白（gp 78，gp 70和gp 32）和一种细胞外物质（gp 70），它们来源于糖皮质激素处理的野生型细胞中的gPr 74 env，未能出现在CR 4中。CR 4的增殖率、细胞形状或总功能mRNA和大量糖蛋白的表达没有明显变化。我们的结论是，CR 4中的遗传病变定义了一个高度选择性的激素调节的糖蛋白成熟途径，改变了前体物种的一个有限的子集的命运。
Glucocorticoid hormones can regulate the posttranslational maturation of mouse mammary tumor virus (MTV) precursor polyproteins in M1.54, a stably infected rat hepatoma cell line. We have used complement- mediated cytolysis to recover variants of M1.54 that fail to express MTV cell surface glycoproteins in a hormone-regulated manner (Firestone, G.L., and K.R. Yamamoto, 1983, Mol. Cell. Biol., 3:149- 160). One such clonal isolate, CR4, is similar to wild-type with respect to synthesis of MTV mRNAs, production of the MTV glycoprotein precursor (gPr74env) and a glycosylated maturation product (gp51), and hormone-induced processing of two MTV phosphoproteins. In contrast, three viral cell surface glycoproteins (gp78, gp70, and gp32) and one extracellular species (gp70s), which derive from gPr74env in glucocorticoid-treated wild-type cells, fail to appear in CR4. CR4 showed no apparent alterations in proliferation rate, cell shape, or expression of total functional mRNA and bulk glycoproteins. We conclude that the genetic lesion in CR4 defines a highly selective hormone- regulated glycoprotein maturation pathway that alters the fate of a restricted subset of precursor species.