pannier encodes two structurally related isoforms that are differentially expressed during Drosophila development and display distinct functions during thorax patterning
pannier encodes two structurally related isoforms that are differentially expressed during Drosophila development and display distinct functions during thorax patterning
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DOI：
10.1016/j.mod.2007.10.008
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发表时间：
2008-01-01
影响因子：
2.6
通讯作者：
Ramain, Philippe
中科院分区：
文献类型：
--
作者：
Fromental-Ramain, Catherine;Vanolst, Luc;Ramain, Philippe
已有研究表明，果蝇pannier （pnr）基因编码GATA转录因子，该转录因子参与多种生物学过程，包括胚胎发育过程中的心脏发育、背侧闭合以及想象发育过程中的神经发生和无翅（wg）表达调控。我们在这里证明了pnr编码两个高度相关的异构体，它们共享功能域，但在发育过程中表达不同。此外，我们描述了pnr基因座的两个基因组区域，它们驱动转基因果蝇中报告基因的表达，其模式概括了同种异构体表达的基本特征，表明这些区域包含关键的调控元件。这些元件特别包含在胚胎发生和胚胎发育过程中介导十足截瘫（Dpp）信号表达调控的序列。对pnr等位基因的分析表明，在想象发育过程中，pnr同工异构体差异调节了wg和前叶无毛细胞/鳞片（as/sc）靶点的表达。Pnr功能已被证明是激活wg和u形（Ush）的必要条件，也是在假定的nottum的背侧大部分区域抑制wg的必要条件。这两种异构体的表达定义了不同的纵向结构域，在这方面，我们重要地表明，pnr在调节wg过程中的双重功能是通过一种异构体抑制椎间盘背侧大部分区域形态原的表达，而另一种异构体则在侧面促进wg表达的激活来实现的。我们的研究为果蝇发育过程中pnr的功能提供了新的见解，并扩展了我们对胸腔模式形成过程中模式前因子作用的认识。2007爱思唯尔爱尔兰有限公司版权所有。
Previous studies have shown that the pannier (pnr) gene of Drosophila encodes a GATA transcription factor which is involved in various biological processes, including heart development, dorsal closure during embryogenesis as well as neurogenesis and regulation of wingless (wg) expression during imaginal development. We demonstrate here that pnr encodes two highly related isoforms that share functional domains but are differentially expressed during development. Moreover, we describe two genomic regions of the pnr locus that drive expression of a reporter in transgenic flies in patterns that recapitulate essential features of the expression of the isoforms, suggesting that these regions encompass crucial regulatory elements. These elements contain, in particular, sequences mediating regulation of expression by Decapentaplegic (Dpp) signaling, during both embryogenesis and imaginal development. Analysis of pnr alleles reveals that the isoforms differentially regulate expression of both wg and proneural achaete/scute (as/sc) targets during imaginal development. Pnr function has been demonstrated to be necessary both for activation of wg and, together with U-shaped (Ush), for its repression in the dorsal-most region of the presumptive notum. Expression of the isoforms define distinct longitudinal domains and, in this regard, we importantly show that the dual function of pnr during regulation of wg is achieved by one isoform repressing expression of the morphogen in the dorsal-most region of the disc while the other laterally promotes activation of the notal wg expression. Our study provides novel insights into pnr function during Drosophila development and extends our knowledge of the roles of prepattern factors during thorax patterning. (c) 2007 Elsevier Ireland Ltd. All rights reserved.