Identification and molecular characterization of non-polio enteroviruses from children with acute flaccid paralysis in West Africa, 2013-2014.
Identification and molecular characterization of non-polio enteroviruses from children with acute flaccid paralysis in West Africa, 2013-2014.
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DOI：
10.1038/s41598-017-03835-1
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发表时间：
2017-06-19
影响因子：
4.6
通讯作者：
Ndiaye K
中科院分区：
文献类型：
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作者：
Fernandez-Garcia MD;Kebe O;Fall AD;Ndiaye K
除脊髓灰质炎病毒外，非脊髓灰质炎肠病毒（npev）也可能与急性弛缓性麻痹（AFP）有关。由于脊髓灰质炎即将被消灭，因此应更加重视研究非脊髓灰质炎AFP病例的npev及其流行模式。在西非国家，npev的流行病学基本上仍未得到探索。我们通过回顾性分析2013年至2014年通过常规脊髓灰质炎监测活动收集的3195例AFP患儿粪便样本，调查了7个西非国家npev的遗传多样性、频率、循环模式和分子流行病学。201株NPEV的VP1测序和分型结果显示，39种NPEV分别为EV-A（6.9%）、EV-B（90.5%）、EV-C（2%）和EV-D（0.5%）。分离最多的是埃可病毒138例（68.6%），其次是柯萨奇病毒B组35例（17.4%）。没有单一的NPEV类型显著占主导地位。有趣的是，在全球范围内发现了几种很少被描述且检测有限的类型（EVA76、EVA119、EVB75、EVB77、EVB97、EVC99、CVA20、CVA21和EVD94）。这项研究显示了AFP监测中NPEV的广泛多样性和不同的传播模式，并强调了制定有效的长期战略来监测西非NPEV传播的必要性。
Besides polioviruses, non-polio enteroviruses (NPEVs) may also be associated with acute flaccid paralysis (AFP). Because poliomyelitis is on the verge of eradication, more attention should be paid to study NPEVs from non-polio AFP cases and their epidemic patterns. In West African countries the epidemiology of NPEVs remains largely unexplored. We investigated the genetic diversity, frequency, circulation patterns, and molecular epidemiology of NPEVs in seven West African countries by analyzing retrospectively a panel of 3195 stool samples from children with AFP collected through routine poliomyelitis surveillance activities between 2013 and 2014. VP1 sequencing and typing on 201 isolates revealed 39 NPEV types corresponding to EV-A (6.9%), EV-B (90.5%), EV-C (2%) and EV-D (0.5%) species. Echoviruses were isolated most frequently with 138 cases (68.6%), followed by coxsackievirus group B with 35 cases (17.4%). No single NPEV type was remarkably dominant. Interestingly, several rarely described types with limited detection worldwide were identified (EVA76, EVA119, EVB75, EVB77, EVB97, EVC99, CVA20, CVA21 and EVD94). This study demonstrates the extensive diversity and diverse circulation patterns of NPEVs from AFP surveillance and highlights the need to formulate effective long-term strategies to monitor NPEV circulations in West Africa.