The excimer fluorescence of tryptophan, tyrosine and d‐glyceraldehyde‐3‐phosphate dehydrogenase
The excimer fluorescence of tryptophan, tyrosine and d‐glyceraldehyde‐3‐phosphate dehydrogenase
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色氨酸、酪氨酸和 d-甘油醛-3-磷酸脱氢酶的准分子荧光
DOI：
10.1016/0014-5793(70)80181-8
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发表时间：
1970
期刊：
影响因子：
3.5
通讯作者：
T. Keleti
中科院分区：
文献类型：
--
作者：
T. Keleti
分析不同浓度下酪氨酸和色氨酸的荧光。浓度范围为 10-50 pg/ml 的酪氨酸显示出 305 至 3·12 mn 之间的最大荧光（取决于浓度），最大激发波长为 283 至 290 nm。在较高浓度下，表现出自猝灭作用，并且这种荧光在 10 mg/ml 时几乎无法测量。然而，在 330 至 340 nm 之间激发酪氨酸浓溶液 (1-10 mg/ml)，并测量 405 至 420 nm 之间的荧光时，发现了新的荧光（图 1）。色氨酸在 lo-50 pg/ml 时的激发最大值落在 290 至 295 nm 之间，荧光最大值在 360 nm 处。当使用1-5mg/ml色氨酸时，只有在308-314nm激发时才能测量360nm处的荧光。与此同时，出现了一条新的荧光带，其激活最大值位于 385 nm，荧光最大值位于 450 nm（图 2）。
The fluorescence of tyrosine and tryptophan was analyzed at different concentrations. Tyrosine in the concentration range of lo-50 pg/ml shows a fluoref cence maximum between 305 and 3 12 mn (depending on concentration) with an excitation maximum between 283 and 290 nm. At higher concentrations, self-quenching was demonstrated and this fluorescence becomes practically unmeasurable at 10 mg/ml. However, a new fluorescence was found on exciting a concentrated solution of tyrosine (1-10 mg/ml) between 330 and 340 nm, and measuring the fluorescence between 405 and 420 nm (fig. 1).The excitation maximum of tryptophan, at lo-50 pg/ml, falls between 290 and 295 nm with a fluorescence maximum at 360 nm. When l-5 mg/ml tryptophan was used, the fluorescence at 360 nm could only be measured if excited at 308-314 nm. Concomitantly, a new fluorescence band appears with activation maximum at 385 nm and fluorescence maximum at 450 nm (fig. 2).