Protein characterization ofBabesia equi piroplasms isolated from infected horse erythrocytes
Protein characterization ofBabesia equi piroplasms isolated from infected horse erythrocytes
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从受感染的马红细胞中分离出的马巴贝斯虫梨原体的蛋白质特征
DOI：
10.1007/bf00932505
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发表时间：
2004
影响因子：
2
通讯作者：
M. Kamada
中科院分区：
文献类型：
--
作者：
S. Ali;C. Sugimoto;M. Matsuda;T. Sugiura;T. Kanemaru;M. Onuma;M. Kamada
巴贝斯虫装备质体的蛋白质特征。b。用氮气空化裂解感染马红细胞，经Percoll密度梯度离心分离纯化。经二维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的主要蛋白的相对分子量（Mr）分别为18、28、30、41、43、54、66.5和96 kDa。利用实验感染马的血清进行免疫印迹分析，发现6种免疫优势蛋白，分别为15、18、28、30、41和96 kDa。Triton X-114相分配显示，两个免疫优势蛋白为18和28 kDa，是膜结合蛋白。
Proteins ofBabesia equipiroplasms were characterized. The piroplasms ofB. equiwere purified by lysis of infected horse erythrocytes with N2gas cavitation followed by separation in Percoll density-gradient centrifugation. The relative molecular weights (Mr) of major proteins separated by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis were 18, 28, 30, 41, 43, 54, 66.5, and 96 kDa. Immunoblot analysis using serum from an experimentally infected horse revealed six immunodominant proteins of 15, 18, 28, 30, 41, and 96 kDa. Two immunodominant proteins of 18 and 28 kDa were membrane-bound proteins as revealed by Triton X-114 phase partitioning.