CHARACTERISTICS OF LYMPHOCYTE-T CELL-LINES ESTABLISHED FROM NIH MINIPIGS CHALLENGE INOCULATED WITH TRICHINELLA-SPIRALIS
CHARACTERISTICS OF LYMPHOCYTE-T CELL-LINES ESTABLISHED FROM NIH MINIPIGS CHALLENGE INOCULATED WITH TRICHINELLA-SPIRALIS
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DOI：
10.1016/0165-2427(93)90041-2
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发表时间：
1993-01-01
影响因子：
1.8
通讯作者：
LUNNEY, JK
中科院分区：
文献类型：
--
作者：
DILLENDER, MJ;LUNNEY, JK
MHC受限制的抗原特异性，细胞因子依赖的T细胞系是由外周血单核细胞（PBMC）建立的NIH Minipigs的，这些细胞是被毛状螺旋中接种的挑战。 SLA（A/A）（AA）单倍型的猪淋巴细胞抗原（SLA）单型NIH Minipigs可以根据其破坏生成的肌肉幼虫的能力，将其分为响应者（R）和非反应器（NR）表型）寄生虫T螺旋形的形式。当口服300个感染性ML并接种10 000 mL的挑战时，R Pigs能够在初次接种以来对宿主肌肉细胞中驻留在宿主肌肉细胞中的百年型ML的免疫发作。由于T-淋巴细胞介导的机制可能在此反应中可以操作，因此在挑战感染T Spiralis后1 0天从PBMC建立了一系列T淋巴细胞细胞系。这些T淋巴细胞线显示出抗原特异性，细胞因子依赖性增殖，以响应T. spiralis抗原刺激。比较了来自R和NR猪的T淋巴细胞细胞系，发现在增生反应的大小，抗原特异性的持续时间以及抗原刺激后T-淋巴细胞亚群和细胞表面表面表面表面表面表面表面的改变中相似。 T-淋巴细胞细胞系的体外抗原刺激以及通过密度梯度离心，免疫荧光染色和流式细胞术评估小淋巴细胞和淋巴细胞的评估，表明多达55％的细胞是在刺激后4天的55％的细胞。淋巴细胞种群表达CD4和CD8，以及白介素-2受体和II类同种抗原Sla-dr（DR +）。这些结果表明，CD4+ CD8+表型的猪T淋巴细胞可能参与了吞噬细胞介导的对T. spiralis的免疫反应。
MHC restricted, antigen specific, cytokine dependent T-cell lines were established from peripheral blood mononuclear cells (PBMC) of NIH minipigs that were challenge inoculated with Trichinella spiralis. Swine lymphocyte antigen (SLA) inbred NIH minipigs of the SLA(a/a) (aa) haplotype can be divided into Responder (R) and Non-responder (NR) phenotypes on the basis of their ability to destroy encysted muscle larval (ML) forms of the parasite T spiralis. When orally inoculated with 300 infectious ML and challenge inoculated with 10 000 ML, R pigs are able to mount an immune attack against encysted ML resident in host muscle cells since the primary inoculation. Because T-lymphocyte mediated mechanisms are likely to be operative in this response, a series of T-lymphocyte cell lines was established from PBMC of ten aa minipigs 1 0 days after the challenge infection with T spiralis. These t-lymphocyte lines showed antigen specific, cytokine dependent proliferation in response to T. spiralis antigen stimulation. T-lymphocyte cell lines from R and NR pigs were compared and found to be similar in the magnitude of proliferative responses, in the duration of antigen specificity, and in the alterations in T-lymphocyte subsets and cell surface phenotype after antigen stimulation. In vitro antigen stimulation of T-lymphocyte cell lines and evaluation of small lymphocytes and lymphoblasts by density gradient centrifugation, immunofluorescent staining and flow cytometry, revealed that up to 55% of the cells were lymphoblasts at 4 days after stimulation, and that the majority of the lymphoblast population expressed CD4 and CD8, as well as the interleukin-2 receptor and the Class II alloantigen, SLA-DR (DR + ). These results indicate that pig T-lymphocytes of the CD4+CD8+ phenotype may be involved in the anamnestic cell mediated immune response to T. spiralis.