Predictive value of N-acetylglucosaminyltransferase-V for superficial bladder cancer recurrence
Predictive value of N-acetylglucosaminyltransferase-V for superficial bladder cancer recurrence
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DOI：
10.1016/s0022-5347(05)00044-3
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发表时间：
2006-01-01
影响因子：
6.6
通讯作者：
Ohyama, C
中科院分区：
文献类型：
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作者：
Takahashi, T;Hagisawa, S;Ohyama, C
目的：GnT-V是一种催化细胞蛋白天冬酰胺（N）连接寡糖上N-乙酰葡糖胺β 1-6分支的酶。GnT-V的表达与结肠癌、脑癌和肝细胞癌的恶性潜能密切相关。我们确定是否GnT-V的表达是预测浅表性膀胱癌recurrence.Materials和方法：该队列包括60例连续的第一次浅表性膀胱癌经尿道切除术治疗。所有患者均未接受预防性膀胱灌注。治疗直至复发。石蜡包埋的肿瘤标本采用抗GnT-V单克隆抗体，通过抗生物素蛋白-生物素过氧化物酶法进行免疫组化检查。Kaplan-Meier生存曲线用于确定无病生存期。单因素和多因素分析比较GnT-V表达与其他临床和病理变量。结果：GnT-V表达与肿瘤分级和分期呈负相关。GnT-V在G1 ~ G3期肿瘤中的阳性率分别为7/9（78%）、21/43（49%）和38（38%）。GnT-V在44例pTa中26例（60%）和16例pT 1中5例（31%）阳性。GnT-V表达阳性的31例患者的无病生存率显著高于GnT-V表达阴性的29例患者（对数秩检验p = 0.0034）。多因素分析显示患者年龄、pT、分级和GnT-V阴性表达是复发的独立预测因子（p值分别为0.015、0.001、0.019和0.011）。结论：GnT-V的免疫组化检测是浅表性膀胱癌复发的独立预测因子。
Purpose: GnT-V is an enzyme that catalyzes beta 1-6 branching of N-acetylglucosamine on asparagine (N)-linked oligosaccharides of cell proteins. GnT-V expression has been closely related to malignant potentials in colon cancer, brain cancer and hepatocellular carcinoma. We determined whether GnT-V expression is predictive of superficial bladder cancer recurrence.Materials and Methods: The cohort comprised 60 consecutive patients with first time superficial bladder cancer treated with transurethral resection. None of the patients received prophylactic intravesical. therapy until recurrence. Paraffin embedded tumor specimens were immunohistochemically examined by the avidin-biotin peroxidase method using monoclonal antibody against GnT-V. Kaplan-Meier survival curves were generated to determine disease-free survival. Univariate and multivariate analyses were done to compare GnT-V expression to other clinical and pathological variables.Results: GnT-V expression correlated inversely with tumor grade and stage. The positive incidence of GnT-V in G1 to G3 tumors was 7 of 9 (78%), 21 of 43 (49%) and 3 of 8 (38%), respectively. GnT-V was positive in 26 of 44 cases of pTa (60%) and in 5 of 16 of pT1 (31%) disease. The 31 patients with positive GnT-V expression had significantly higher disease-free survival than the 29 with negative GnT-V expression (log rank test p = 0.0034). Multivariate analysis revealed that patient age, pT, grade and negative GnT-V expression were independent predictors of recurrence (p = 0.015, 0.001, 0.019 and 0.011, respectively).Conclusions: Immunohistochemical detection of GnT-V is an independent predictor of superficial bladder cancer recurrence.