Characterization of Neisseria meningitidis isolates collected from 1974 to 2003 In Japan by multilocus sequence typing
Characterization of Neisseria meningitidis isolates collected from 1974 to 2003 In Japan by multilocus sequence typing
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DOI：
10.1099/jmm.0.45541-0
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发表时间：
2004-07-01
影响因子：
3
通讯作者：
Watanabe, H
中科院分区：
文献类型：
--
作者：
Takahashi, H;Kuroki, T;Watanabe, H
通过血清群分型和多位点序列分型（MLST）对日本过去30年分离的182株脑膜炎奈瑟菌菌株进行分析。182株日本分离株的血清群为B（103株）、Y（39株）、W135（1株）和不可分组（39株）。通过MLST分析，鉴定出65种不同的序列类型（ST），其中42种在截至2004年1月的MLST数据库中未发现，似乎是日本独有的。MLST结果的统计分析表明，虽然日本分离株似乎是遗传分歧，他们被分为6个主要的克隆复合体和其他次要的复合体。在这些分离株中，存在世界范围内已被充分记录的ST复合体，如ST-23复合体（49株）、ST-44复合体（41株）和ST-32复合体（8株）。另一方面，还发现了一个新的克隆复合体，命名为ST-2046复合体（28个分离物），这在其他国家还没有被确定，这表明该克隆是日本本土的。综上所述，推测日本的脑膜炎球菌分离株包含异质克隆，这些克隆来自其他国家鉴定的克隆和日本特有的克隆。
Analysis of 182 Neisseria meningitidis strains isolated over the past 30 years in Japan by serogroup typing and multilocus sequence typing (MLST) was performed. The serogroups of the 182 Japanese isolates were B (103 isolates), Y (39), W135 (1) and non-groupable (39). By MLST analysis, 65 different sequence types (ST) were identified, 42 of which were not found in the MLST database as of January 2004 and seemed to be unique to Japan. Statistical analysis of the MLST results revealed that, although the Japanese isolates seemed to be genetically divergent, they were classified into six major clonal complexes and other minor complexes. Among these isolates, well-documented ST complexes found worldwide were present, such as ST-23 complex (49 isolates), ST-44 complex (41 isolates) and ST-32 complex (8 isolates). On the other hand, a new clonal complex designated ST-2046 complex (28 isolates), which has not been identified in other countries, was also found, suggesting that this clone was indigenous to Japan. Taken together, it was speculated that meningococcal isolates in Japan comprised heterogeneous clones, which were derived both from clones identified in other countries and clones unique to Japan.