A gene coding for a ribosomal protein L41 in cycloheximide-resistant ribosomes has a promoter which is upregulated under the growth-inhibitory conditions in yeast, Candida maltosa.
A gene coding for a ribosomal protein L41 in cycloheximide-resistant ribosomes has a promoter which is upregulated under the growth-inhibitory conditions in yeast, Candida maltosa.
复制标题
放线菌酮抗性核糖体中编码核糖体蛋白 L41 的基因具有一个启动子，该启动子在麦芽糖酵母的生长抑制条件下上调。
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
M. Takagi
中科院分区：
文献类型：
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作者：
E. Mutoh;H. Takaku;A. Ohta;M. Takagi
我们之前通过使用酵母菌麦芽糖发现，核糖体的放线菌酮 (CYH) 敏感性取决于核糖体蛋白 L413 的第 56 个氨基酸残基（敏感核糖体中为脯氨酸，抗性核糖体中为谷氨酰胺）。我们还发现，在这种同时具有L41-P型和L41-Q型基因的酵母中，通过在培养基中添加CYH来诱导后一型基因的表达，从而使细胞对CYH产生诱导性抗性。在本文中，我们分析了CYH诱导型L41-Q型基因之一的L41-Q2a的启动子区域，发现了诱导表达所需的两个元件：一个是GCRE（酿酒酵母的Gcn4p响应元件）样元件，另一个是富含GT的元件。在一些其他生长抑制条件下的表达也需要该启动子区域。此外，有人认为在这些条件下合成的 Q 型核糖体对这些抑制条件更具抵抗力。
We previously found by using yeast, Candida maltosa, that cycloheximide (CYH) sensitivity of ribosomes is dependent on the 56th amino acid residues of a ribosomal protein, L413 (proline in sensitive and glutamine in resistant ribosomes). We also revealed that in this yeast, which has both L41-P type and L41-Q type genes, the expression of the latter type genes is induced by the addition of CYH in the medium to make the cells inducibly resistant to CYH. In this paper, we analyzed the promoter region of L41-Q2a, one of the CYH-inducible L41-Q type genes and found two elements required for the induction of expression: one was a GCRE (Gcn4p-responsive element of Saccharomyces cerevisiae)-like element and the other was a GT-rich element. This promoter region was also required for its expression under some other growth inhibitory conditions. Furthermore, it was suggested that Q-type ribosomes synthesized under these conditions are more resistant to these inhibitory conditions.
影响因子：
11.4
作者：
IYER, V;STRUHL, K
通讯作者：
STRUHL, K