Structure and organization of the rrnD operon of 'Brevibacterium lactafermentum':: analysis of the 16S rRNA gene
Structure and organization of the rrnD operon of 'Brevibacterium lactafermentum':: analysis of the 16S rRNA gene
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DOI：
10.1099/13500872-145-4-915
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发表时间：
1999-04-01
期刊：
影响因子：
2.8
通讯作者：
Martín, JF
中科院分区：
文献类型：
--
作者：
Amador, E;Castro, JM;Martín, JF
通过杂交，在乳酸短杆菌ATCC 13869和谷氨酸棒杆菌ATCC 13032基因组中发现了5个rRNA操纵子。‘B.lactofermentum’DSM 20412与其他供试棒状菌不同，表现出6条杂交的BamHI条带。两个Rm操纵子(rrnD和rrnE)位于单个粘粒中。RrnD操纵子序列分析表明，该操纵子包含一个完整的165 rRNA-23S RNA-5S rRNA基因簇。利用165rRNA全序列进行的系统发育研究表明，B.lactofermentum与棒状杆菌属的几个种有较近的亲缘关系，但与模式种亚麻短杆菌有较远的亲缘关系，作者建议将其重新归类为乳酸发酵棒杆菌。扩增得到成熟的165rRNA的5‘端。鉴定了与参与转录抗终止(框A、B、C)和将Pre-rRNA加工到165rRNA的分支杆菌相似的序列元件。在rrnD的上游发现了一个与已报道的SIgA和SigB蛋白不同的编码rpoD样sigma因子的开放阅读框(命名为SigC)。RpoD和sigC似乎都是从双向启动子区域表达的。
Five rRNA operons (rm) were found by hybridization in the genome of 'Brevibacterium lactofermentum' ATCC 13869 and Corynebacterium glutamicum ATCC 13032. 'B. lactofermentum' DSM 20412 differed from the other corynebacteria tested in showing six hybridizing BamHI bands. Two of the rm operons (rrnD and rrnE) were located in a single cosmid. Sequencing of the rrnD operon showed that it contains a complete 165 rRNA-23S RNA-5S rRNA gene cluster. Phylogenetic studies using the complete 165 rRNA sequence showed that 'B. lactofermentum' is closely related to several species of the genus Corynebacterium but only distantly related to the type species Brevibacterium linens and the authors suggest that it should be reclassified as Corynebacterium lactofermentum. The 5' end of mature 165 rRNA was identified by primer extension. Sequence elements similar to those of mycobacteria implicated in transcription antitermination (Boxes A, B, C) and in processing of the pre-rRNA to 165 rRNA were identified. An open reading frame encoding an rpoD-like sigma factor (named SigC) different from the previously reported SigA and SigB proteins was found upstream of rrnD in the opposite orientation. Both rpoD and sigC seem to be expressed from a bidirectional promoter region.