Characterization of Extended-Spectrum Beta-lactamase from Escherichia coli and Klebsiella Species from North Eastern Nigeria
Characterization of Extended-Spectrum Beta-lactamase from Escherichia coli and Klebsiella Species from North Eastern Nigeria
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DOI：
10.7860/jcdr/2016/16330.7254
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发表时间：
2016-02-01
影响因子：
0.2
通讯作者：
Aboderin, Aaron Oladipo
中科院分区：
文献类型：
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作者：
Mohammed, Yahaya;Gadzama, Galadima Bala;Aboderin, Aaron Oladipo
简介：抗菌药物耐药性已成为一个严重的全球健康问题，使治疗策略复杂化并增加医疗费用。产生超广谱β-内酰胺酶的细菌是革兰氏阴性杆菌中备受关注的流行病细菌。对抗菌药物耐药性的控制和适当的干预措施取决于有效的监测和对耐药性模式和决定因素的了解。目的：本研究旨在检测和表征尼日利亚东北部迈杜古里迈杜古里大学教学医院的大肠杆菌和克雷伯氏菌中的 ESBL。材料和方法：从各个单位收治的 439 名患者中分离出已确认的大肠杆菌和克雷伯氏菌变种使用 CLSI 断点对迈杜古里大学教学医院 (UMTH) 的 ESBL 进行了筛查。采用双盘协同法对疑似ESBLs生产者进行了确认。通过多重PCR进一步检测ESBL基因。结果：筛选出439株分离株；结果显示147个（33.5%）是ESBL产生者，但通过双盘协同法仅确认了121个（23.6%）。生物体中 ESBL 的流行率为：大肠杆菌为 41/172 (23.8%)，克雷伯氏菌为 80/267/(30.0%)。基于PCR分析，ESBL产生者的不同基因型百分比为SHV基因44(36.4%)，其次是TEM基因38(31.4%)，CTX-M基因最低为33(27.3%)。结论：博尔诺州迈杜古里的大肠杆菌和克雷伯菌中ESBLs普遍存在，不仅存在TEM和SHV，而且存在ESBLs。还有 CTX-M 类型。强调了最大限度地利用现有抗生素的抗生素管理计划，以及协调国家抗击耐药性的努力。
Introduction: Resistance to antimicrobials has become a serious global health concern complicating treatment strategies and increasing health-care costs. The extended-spectrum beta-lactamase producing bacteria stand out as bacteria of great epidemic concern among Gram negative bacilli. Control and appropriate interventions for antimicrobial resistance depend on effective surveillance and knowledge of the patterns and determinants of resistance.Aim: The present study was undertaken to detect and characterize ESBLs in Escherichia coli and Klebsiella Species from University of Maiduguri Teaching Hospital, Maiduguri, North-Eastern Nigeria.Materials and Methods: Confirmed variants of Escherichia coli and Klebsiella Species isolated from 439 patients that were admitted in various units of University of Maiduguri Teaching Hospital (UMTH) were screened for ESBL using CLSI breakpoints. Suspected ESBLs producers were subjected to confirmation using double disk synergy method. Detection of ESBL genes was further done by multiplex PCR.Results: Out of the 439 isolates screened; the result shows 147 (33.5%) were ESBL producers but only 121(23.6%) were confirmed by the double disk synergy method. The prevalence of ESBL amongst the organisms were; 41/172 (23.8%) for Escherichia coli and 80/267/(30.0%) for Klebsiella Species. Based on PCR analysis, the various percentage genotypes of the ESBL producers were 44 (36.4%) for SHV gene followed by 38(31.4%) for TEM gene and the lowest of 33(27.3%) for CTX-M gene.Conclusion: ESBLs are prevalent among Species of Escherichia coli and Klebsiella Species in Maiduguri, Borno State, not only are there TEM and SHV but also CTX-M types. Antibiotic stewardship program to maximise use of available antibiotics is underscored as well as coordinated national efforts in combating resistance.