Influence of coumarin and some coumarin derivatives on serum lipid profiles in carbontetrachloride-exposed rats
Influence of coumarin and some coumarin derivatives on serum lipid profiles in carbontetrachloride-exposed rats
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DOI：
10.1177/0960327116649675
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发表时间：
2017-03-01
影响因子：
2.8
通讯作者：
Oflazoglu, Hueda Diken
中科院分区：
文献类型：
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作者：
Tasdemir, Ezel;Atmaca, Mukadder;Oflazoglu, Hueda Diken
在本研究中，香豆素及其部分香豆素衍生物(秦皮乙素、滨蒿内酯和4-甲基伞形酮)对大鼠的降脂作用进行了研究。雄性SD大鼠150~200g，随机分为6组，每组5只。用四氯化碳(CCl4，1.25ml/kg)诱导大鼠肝损伤相关性高脂血症。各实验组分别给予香豆素及香豆素衍生物秦皮乙素(35 mg/kg)、东莨菪内酯(35 mg/kg)、4-甲基伞形酮(35 mg/kg)或香豆素(30 mg/kg)，间隔12h。动物在注射单次毒性剂量的CCl4之前，接受了秦皮乙素、东方酮或4-甲基伞形酮的衍生物。CCl4治疗组大鼠血清总胆固醇(TC)、甘油三酯(TG)、极低密度脂蛋白胆固醇(VLDL-C)和低密度脂蛋白胆固醇(LDL-C)水平显著升高(p<；0.05，p<；0.01，p<；0.01和p<；0.05)，而血清高密度脂蛋白胆固醇(HDL-C)水平下降(p<；0.01)。4-甲基伞形酮对血清TC水平无恢复作用，但可通过降低甘油三酯和极低密度脂蛋白胆固醇水平而显著预防CCl_4诱导的高脂血症(p<；0.05和p<；0.05)。此外，香豆素对CCl4诱导的高脂血症的所有血脂指标均无恢复作用。在香豆素衍生物中，只有秦皮乙素和滨藜内酯能显着预防CCl4诱导的血脂异常时的血清高密度脂蛋白胆固醇。本研究结果表明，香豆素类化合物的化学结构在调节血脂谱中起着重要作用。
In the present study, coumarin and some coumarin derivatives (esculetin, scoparone, and 4-methylumbelliferone) were investigated for their lipid-lowering effect in rats. Male Sprague-Dawley rats (150-200 g) were divided into six groups and each group comprised of five rats. Hepatic injury-dependent hyperlipidemia was induced by carbon tetrachloride (CCl4, 1.25 ml/kg). Coumarin and coumarin derivatives esculetin (35 mg/kg), scoparone (35 mg/kg), 4-methylumbelliferone (35 mg/kg), or coumarin (30 mg/kg) were administered to experimental groups at 12-h intervals. Animals received the derivatives esculetin, scoparone or 4-methylumbelliferone prior to the administration of a single toxic dose of CCl4. Serum total cholesterol (TC), triglyceride (TG), very low-density lipoprotein cholesterol (VLDL-C), and low-density lipoprotein cholesterol (LDL-C) levels significantly increased in CCl4-treated group (p < 0.05, p < 0.01, p < 0.01, and p < 0.05, respectively), while levels of serum high-density lipoprotein cholesterol (HDL-C) decreased (p < 0.01). 4-Methylumbelliferone had no recovery effects on serum TC levels, however, significantly prevented CCl4-induced hyperlipidemia by reducing TG and VLDL-C levels (p < 0.05 and p < 0.05, respectively). In addition, coumarin had no recovery effect on any of the serum lipid parameters against CCl4-induced hyperlipidemia. Among the coumarin derivatives only esculetin and scoparone significantly prevented serum HDL-C in CCl4-induced dyslipidemia. The results from this study indicate that the chemical structure of coumarins plays an important role on the regulation of serum lipid profiles.