Microindentation Technique to Create Localized Cartilage Microfractures
Microindentation Technique to Create Localized Cartilage Microfractures
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DOI：
10.1002/cpz1.280
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发表时间：
2021-10
期刊：
影响因子：
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通讯作者：
D. Chawla;G. Han;M. Eriten;C. Henak
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文献类型：
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作者：
D. Chawla;G. Han;M. Eriten;C. Henak
关节软骨是一种多相、各向异性和非均质材料。尽管软骨具有优异的力学和生物学特性，但它也会遭受机械损伤，导致骨关节炎。因此，在基础科学和临床环境中，了解软骨的微尺度失效行为是很重要的。确定软骨衰竭行为和机制为改善治疗策略提供了见解，以延缓骨关节炎的发生或进展，也可以提高软骨作为材料制造的生物灵感的价值。为了研究微尺度的破坏行为，我们开发了一种方案，通过应用微压痕技术，使用定义良好的尖端几何形状，在一定的加载速率范围内产生局部裂纹。该方案包括从关节中提取组织，制备样品和微骨折。实验的各个方面，如负载剖面和溶剂，可以调整以模拟生理或病理条件，从而进一步阐明关节软骨衰竭的现象。©2021 Wiley期刊有限责任公司
Articular cartilage is a multiphasic, anisotropic, and heterogeneous material. Although cartilage possesses excellent mechanical and biological properties, it can undergo mechanical damage, resulting in osteoarthritis. Thus, it is important to understand the microscale failure behavior of cartilage in both basic science and clinical contexts. Determining cartilage failure behavior and mechanisms provides insight for improving treatment strategies to delay osteoarthritis initiation or progression and can also enhance the value of cartilage as bioinspiration for material fabrication. To investigate microscale failure behavior, we developed a protocol to initiate fractures by applying a microindentation technique using a well‐defined tip geometry that creates localized cracks across a range of loading rates. The protocol includes extracting the tissue from the joint, preparing samples, and microfracture. Various aspects of the experiment, such as loading profile and solvent, can be adjusted to mimic physiological or pathological conditions and thereby further clarify phenomena underlying articular cartilage failure. © 2021 Wiley Periodicals LLC.