Intake of water with high levels of dissolved hydrogen (H2) suppresses ischemia-induced cardio-renal injury in Dahl salt-sensitive rats.
Intake of water with high levels of dissolved hydrogen (H2) suppresses ischemia-induced cardio-renal injury in Dahl salt-sensitive rats.
复制标题
DOI：
10.1093/ndt/gfq727
复制
发表时间：
2011-07
期刊：
影响因子：
--
通讯作者：
Wanjun Zhu;M. Nakayama;Takefumi Mori;Keisuke Nakayama;Junichiro Katoh;Yaeko Murata;Toshinobu Sato;S. Kabayama;S. Ito
中科院分区：
文献类型：
--
作者：
Wanjun Zhu;M. Nakayama;Takefumi Mori;Keisuke Nakayama;Junichiro Katoh;Yaeko Murata;Toshinobu Sato;S. Kabayama;S. Ito
据报道，氢（H（2））通过淬灭细胞毒性氧自由基而产生抗氧化作用。我们研究了溶解有H2的水对慢性肾脏病（CKD）大鼠模型缺血诱导的心肾损伤的生物学效应。方法7周龄的Dahl盐敏感大鼠自由饮用过滤水（FW：溶解H2，0.00 ± 0.00 mg/L）或电解产生的溶解H2水（EW：溶解H2，0.35 ± 0.03 mg/L），连续6周，给予0.5%的食盐饮食。然后对大鼠一侧肾脏进行缺血再灌注（I/R），一周后处死，研究对侧肾脏和心脏。结果FW组大鼠肾缺血再灌注后血浆单核细胞趋化蛋白-1、丙酮醛和血尿素氮明显升高。在组织学上，发现对侧肾脏和心脏中的肾小球粘附、心脏纤维化、艾德-1（CD 68）阳性细胞数量和硝基酪氨酸染色显著增加。在给予EW的大鼠中，这些结果显著改善，并且给予FW的大鼠与给予EW的大鼠之间存在显著的组织学差异。结论自由饮用EW可改善CKD模型大鼠缺血性心肾损伤。这表明了一种新的策略，即应用水电解技术产生的H（2）预防CKD心肾综合征。
BACKGROUND Hydrogen (H(2)) reportedly produces an antioxidative effect by quenching cytotoxic oxygen radicals. We studied the biological effects of water with dissolved H(2) on ischemia-induced cardio-renal injury in a rat model of chronic kidney disease (CKD). METHODS Dahl salt-sensitive rats (7 weeks old) were allowed ad libitum drinking of filtered water (FW: dissolved H(2), 0.00 ± 0.00 mg/L) or water with dissolved H(2) produced by electrolysis (EW: dissolved H(2), 0.35 ± 0.03 mg/L) for up to 6 weeks on a 0.5% salt diet. The rats then underwent ischemic reperfusion (I/R) of one kidney and were killed a week later for investigation of the contralateral kidney and the heart. RESULTS In the rats given FW, unilateral kidney I/R induced significant increases in plasma monocyte chemoattractant protein-1, methylglyoxal and blood urea nitrogen. Histologically, significant increases were found in glomerular adhesion, cardiac fibrosis, number of ED-1 (CD68)-positive cells and nitrotyrosine staining in the contralateral kidney and the heart. In rats given EW, those findings were significantly ameliorated and there were significant histological differences between rats given FW and those given EW. CONCLUSION Consumption of EW by ad libitum drinking has the potential to ameliorate ischemia-induced cardio-renal injury in CKD model rats. This indicates a novel strategy of applying H(2) produced by water electrolysis technology for the prevention of CKD cardio-renal syndrome.