Association of TP53 codon 72 polymorphism with cervical cancer risk in Chinese women
Association of TP53 codon 72 polymorphism with cervical cancer risk in Chinese women
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TP53密码子72多态性与中国女性宫颈癌风险的关联。
DOI：
10.1016/j.cancergencyto.2009.11.011
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发表时间：
2010-03-01
影响因子：
--
通讯作者：
Tang, Jianxin
中科院分区：
文献类型：
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作者：
Jiang, Pei;Liu, Jianxin;Tang, Jianxin
TP53 密码子 72 的多态性导致 p53 (R72P) 的精氨酸 (Arg) 或脯氨酸 (Pro) 形式，与不同癌症的易感性相关。为了更好地了解这种多态性在宫颈癌病因学中的作用，我们研究了 p53 R72P 与中国南方汉族女性宫颈癌风险之间的关联。收集了 105 名患有宫颈癌的女性和 140 名女性献血者的血液样本。提取外周血淋巴细胞DNA，聚合酶链式反应扩增TP53外显子4，BstUI酶切。我们观察到，Arg/Arg 基因型与宫颈癌风险增加显着相关[比值比 (OR) = 1.465，95% 置信区间 (95%CI) = 1.04-2.138，P = 0.02]。宫颈癌患者各等位基因的相对频率为Arg 0.69、Pro 0.31，正常对照组Arg 0.60、Pro 0.40（P < 0.05）。此外，我们还发现TP53 R72P多态性与肿瘤组织学类型、组织学分级、淋巴结转移和肿瘤大小无关。这些发现表明，TP53 密码子 72 多态性可能与宫颈癌发生有关，而 Arg/Arg 基因型与中国南方人群对这种恶性肿瘤的易感性增加有关。 (C) 2010 Elsevier Inc. 保留所有权利。
Polymorphism at codon 72 of TP53, resulting in either the arginine (Arg) or proline (Pro) form of p53 (R72P), has been associated with the susceptibility to different cancers. To better understand the role of this polymorphism in cervical cancer etiology, we investigated the association between p53 R72P and cervical cancer risk in Chinese women from southern Han. Blood samples from 105 women with cervical cancer and from 140 female blood donors were collected. DNA from peripheral blood lymphocytes was extracted, and the exon 4 of TP53 was amplified by polymerase chain reaction and then digested by BstUI. We observed that the Arg/Arg genotype was significantly associated with an increased risk for cervical cancer [odds ratio (OR) = 1.465, 95% confidence interval (95%CI) = 1.04-2.138, P = 0.02]. The relative frequency of each allele was 0.69 for Arg and 0.31 for Pro in patients with cervical cancer, and 0.60 for Arg and 0.40 for Pro in normal controls (P < 0.05). Furthermore, we also found that the TP53 R72P polymorphism was not linked to tumor histologic type, histologic grade, lymph node metastases, and tumor size. These findings suggest that the TP53 codon 72 polymorphism may be implicated in cervical carcinogenesis, with the Arg/Arg genotype being associated with an increased susceptibility for this malignancy in the southern Chinese population. (C) 2010 Elsevier Inc. All rights reserved.