Normobaric Hyperoxygenation Enhances Initial Survival, Regeneration, and Final Retention in Fat Grafting
Normobaric Hyperoxygenation Enhances Initial Survival, Regeneration, and Final Retention in Fat Grafting
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DOI：
10.1097/prs.0000000000000600
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发表时间：
2014-11-01
影响因子：
3.6
通讯作者：
Yoshimura, Kotaro
中科院分区：
文献类型：
--
作者：
Kato, Harunosuke;Araki, Jun;Yoshimura, Kotaro
背景：脂肪移植是一种很有前途的软组织增强/重建方式。然而，移植的脂肪组织最初不是灌注的，而是依赖于来自受体床的血浆扩散，直到血运重建发生。作者评估了常压高氧对增强脂肪移植保留的治疗效果。方法：将抽吸的人脂肪组织在组织缺氧（1%氧气）、常氧（6%）和高氧（20%）水平下培养，并评估脂肪细胞活力。将腹股沟脂肪垫自体移植到小鼠头皮下（n = 36），小鼠在20%（对照组）或60%（常压高氧）的大气中饲养3天，然后返回常压高氧环境。在0、1、2、4、8和12周采集的样本进行免疫组织化学分析，检测脂肪细胞的活力和再生。结果：低氧条件下器官培养脂肪细胞死亡较快；因此，受体组织的高氧可延缓脂肪移植后脂肪细胞的死亡。自体移植的小鼠脂肪组织在12周内经历了从缺血变性到部分再生的动态重塑。常压高氧移植样品在1周和12周时的生存区和植入评分（使用样品重量和脂肪细胞活力计算）分别明显大于对照样品。此外，在常压高氧样品中，脂肪细胞再生（perilipin阳性前脂肪细胞数量）在第4周达到峰值，显著增加。结论：60%氧的常压高氧方案可安全用于脂肪移植后脂肪细胞的存活、再生和最终植入。
Background: Fat grafting is a promising modality for soft-tissue augmentation/reconstruction. However, grafted fat tissue is not initially perfused and relies on plasmatic diffusion from the recipient bed until revascularization occurs. The authors evaluated the therapeutic effects of normobaric hyperoxygenation for enhancing fat graft retention.Methods: Aspirated human fat tissue was cultured under tissue hypoxia (1% oxygen), normoxia (6%), and hyperoxia (20%) levels, and evaluated for adipocyte viability. Inguinal fat pads were autografted under mouse scalps (n = 36), and mice were housed in either 20% (control) or 60% (normobaric hyperoxygenation) atmospheric oxygen for the first 3 days, and then returned to normoxia. Samples harvested at 0, 1, 2, 4, 8, and 12 weeks were analyzed immunohistochemically for adipocyte viability and regeneration.Results: Organ culture adipocytes died more quickly under lower oxygen tensions; thus, hyperoxygenation of recipient tissues may delay adipocyte death after fat grafting. Autografted mouse adipose tissue underwent dynamic remodeling, from ischemic degeneration to partial regeneration, over 12 weeks. Normobaric hyperoxygenation grafted samples showed significantly larger survival zones and engraftment scores (calculated using sample weight and adipocyte viability) at 1 and 12 weeks, respectively, than control samples. In addition, adipocyte regeneration (number of perilipin-positive preadipocytes), which peaked at 4 weeks, was significantly increased in normobaric hyperoxygenation samples.Conclusion: The normobaric hyperoxygenation protocol using 60% oxygen can be safely applied to enhance adipocyte survival, regeneration, and final engraftment after fat grafting.