DISTRIBUTION OF NEURONAL CANNABINOID RECEPTOR IN THE ADULT-RAT BRAIN - A COMPARATIVE RECEPTOR-BINDING AUTORADIOGRAPHY AND INSITU HYBRIDIZATION HISTOCHEMISTRY
DISTRIBUTION OF NEURONAL CANNABINOID RECEPTOR IN THE ADULT-RAT BRAIN - A COMPARATIVE RECEPTOR-BINDING AUTORADIOGRAPHY AND INSITU HYBRIDIZATION HISTOCHEMISTRY
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DOI：
10.1016/0306-4522(92)90409-u
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发表时间：
1992-06-01
期刊：
影响因子：
3.3
通讯作者：
VANDERHAEGHEN, JJ
中科院分区：
文献类型：
--
作者：
MAILLEUX, P;VANDERHAEGHEN, JJ
本文报道了大麻素受体在成年大鼠脑内的神经元分布，结合使用合成精神大麻素配体CP55,940的受体结合放射自显影和使用与大鼠大麻素受体cDNA互补的寡核苷酸探针的原位杂交组织化学。在大脑皮层，尤其是额叶和扣带皮层，在第1层和第6层发现了紧密结合，在大多数锥体和非锥体神经元中都有少量mRNA水平，在中等数量的非锥体神经元中，特别是在第2 - 3层和第v - 6层，mRNA水平很高。海马齿状回分子层染色非常致密，靠近颗粒层的门门神经元数量适中，mRNA水平较高。在阿蒙角，特别是在CA3区，树突层中发现了非常紧密的结合，大多数锥体细胞mRNA水平较低，而中等数量的中间神经元mRNA水平较高。在基底节区，外侧壳核、网状黑质、苍白球和核轴内核的结合非常紧密，内侧壳核和尾状核的结合中等；在伏隔核中有轻微的mRNA水平，纹状体中等神经元中也有轻微到中等的mRNA水平。除了轻度结合外，大多数丘脑下核神经元的mRNA水平均为轻度至中度。黑质致密部和腹侧被盖区未发现结合和mRNA。前嗅核中大多数神经元的mRNA水平轻微至中度结合；鼻中隔，尤其是内侧鼻中隔和布洛卡斜带；杏仁核，尤指基底外侧杏仁核；外侧缰;下丘脑腹内侧核；外侧束间核；中央灰色，耳蜗核背侧；parabrachial核;背侧的桥状被；桥的细胞核;孤束核的近交部分；脊髓的下橄榄角和背角。在小脑中，分子层结合非常紧密，大多数颗粒细胞mRNA水平较低，篮状细胞和星状细胞mRNA水平中等。综上所述，本研究首次对成年大鼠全脑含大麻素受体神经元进行了间接评价，为未来受体免疫组化直接形态学确认和功能研究奠定了基础。
The neuronal distribution of cannabinoid receptor in the adult rat brain is reported, combining receptor binding radioautography using the synthetic psychoative cannabinoid ligand CP55,940 with in situ hybridization histochemistry using oligonucleotide probes complementary to rat cannabinoid receptor cDNA.In the cerebral cortex, especially in the frontal and cingulate cortex, dense binding was found in layers I and VI together with slight mRNA levels in a majority of both pyramidal and non-pyramidal-shaped neurons and of high mRNA levels in a moderate number of non-pyramidal-shaped neurons especially in layers II-III and V-VI. In the hippocampal dentate gyrus, very dense staining was found in the molecular layer together with high mRNA levels in a moderate number of hilar neurons close to the granular layer. In Ammon's horn, especially in the CA3 sector, very dense binding was found in the dendritic layers together with slight mRNA levels in the majority of the pyramidal cells and high mRNA levels in a moderate number of interneurons. In the basal ganglia, binding was very dense in the lateral putamen, substantia nigra pars reticulata, globus pallidus and entopeduncular nucleus, moderate in the medial putamen and caudate; and slight in the accumbens, together with slight to moderate mRNA levels in the striatal medium-sized neurons. Together with slight binding, slight to moderate mRNA levels were found in the majority of the neurons in the subthalamic nucleus. No binding and mRNA were found in the substantia nigra pars compacta and ventral tegmental area. Slight to moderate binding was found together with slight to moderate mRNA levels in the majority of neurons in the anterior olfactory nucleus; septum, especially medial septum and diagonal band of Broca; amygdala, especially basolateral amygdala; lateral habenula; ventromedial hypothalamic nucleus; lateral interpenduncular nucleus; central gray, dorsal cochlear nucleus; parabrachial nucleus; dorsal pontine tegmentum; pontine nuclei; commissural part of the nucleus tractus solitarius; inferior olive and dorsal horn of the spinal cord. In the cerebellum, very dense binding was found in the molecular layer together with slight mRNA levels in the majority of the granule cells and moderate mRNA levels in the basket and stellate cells.In conclusion, this study provides, for the first time, indirect assessment of the neurons containing cannabinoid receptor in the entire adult rat brain and will serve as a basis for future direct morphological confirmation using receptor immunohistochemistry and for functional studies.