Chlamydia trachomatis‐infected human cells convert ceramide to sphingomyelin without sphingomyelin synthases 1 and 2
Chlamydia trachomatis‐infected human cells convert ceramide to sphingomyelin without sphingomyelin synthases 1 and 2
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DOI：
10.1002/1873-3468.13632
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发表时间：
2019-10
期刊：
影响因子：
3.5
通讯作者：
Y. Tachida;Keigo Kumagai;S. Sakai;S. Ando;T. Yamaji;K. Hanada
中科院分区：
文献类型：
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作者：
Y. Tachida;Keigo Kumagai;S. Sakai;S. Ando;T. Yamaji;K. Hanada
专性细胞内细菌沙眼衣原体在宿主细胞形成的膜室包涵体中增殖。宿主神经酰胺转运蛋白CERT将神经酰胺从内质网运送到高尔基复合体以合成鞘磷脂（SM）。沙眼衣原体被认为在宿主SM合酶（SMSs）包涵体中利用CERT产生SM。在这里，我们发现沙眼衣原体在SMS1和SMS2双敲除的HeLa细胞中增殖并产生感染的后代，但在SMS1/SMS2/CERT三敲除的细胞中却没有。有趣的是，受感染的细胞在没有宿主sms的情况下将神经酰胺转化为SM。这些结果表明，沙眼衣原体感染的细胞在CERT介导神经酰胺转移到包涵体后，可以在没有宿主sms的情况下将神经酰胺转化为SM。
The obligate intracellular bacterium Chlamydia trachomatis proliferates in the membranous compartment inclusion formed in host cells. The host ceramide transport protein CERT delivers ceramide from the endoplasmic reticulum to the Golgi complex for the synthesis of sphingomyelin (SM). Chlamydia trachomatis has been suggested to employ CERT to produce SM in the inclusion by host SM synthases (SMSs). Here, we found that C. trachomatis proliferates and produces infective progeny even in SMS1 and SMS2 double‐knockout HeLa cells, but not in the SMS1/SMS2/CERT triple‐knockout cells. Interestingly, infected cells convert ceramide to SM without host SMSs. These results suggest that C. trachomatis‐infected cells can convert ceramide to SM without host SMSs after CERT‐mediated transfer of ceramide to the inclusions.