Immunostimulators induce granulocyte/macrophage colony-stimulating activity and block proliferation in a monocyte tumor cell line
Immunostimulators induce granulocyte/macrophage colony-stimulating activity and block proliferation in a monocyte tumor cell line
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免疫刺激剂诱导粒细胞/巨噬细胞集落刺激活性并阻止单核细胞肿瘤细胞系的增殖
DOI：
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发表时间：
1977
影响因子：
15.3
通讯作者：
I. Nakoinz
中科院分区：
文献类型：
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作者：
Peter Ralph;H. Broxmeyer;I. Nakoinz
单核细胞肿瘤细胞系PU 5 -1.8通常不产生粒细胞和巨噬细胞祖细胞在琼脂中增殖和成熟所需的集落刺激活性（CSA）。然而，CSA在培养系中由低至10 ng/ml的内毒素脂多糖（LPS）诱导，在孵育的2至3天之间具有最大CSA产生和释放到培养基中。衍生的脂质A，但不是碱处理的LPS，也是有活性的。诱导需要RNA和蛋白质合成，但不被丝裂霉素C或秋水仙胺阻断。CSA的其它诱导剂包括分枝杆菌卡介苗、结核菌素蛋白制备纯化蛋白衍生物、酵母聚糖和佛波醇肉豆蔻酸酯。所有诱导剂在体外都是单核细胞肿瘤细胞增殖的特异性抑制剂。另一种巨噬细胞活化剂乳胶珠被PU 5 -1.8细胞迅速吞噬，但既不抑制生长也不诱导CSA。
Monocyte tumor cell line PU5-1.8 does not normally produce colony- stimulating activity (CSA) required by granulocyte and macrophage progenitors to proliferate and mature in agar. However, CSA is induced in the culture line by as little as 10 ng/ml endotoxic lipopolysaccharide (LPS), with maximum CSA production and release to the medium between 2 and 3 days of incubation. Derived lipid A, but not alkali-treated LPS, is also active. Induction requires RNA and protein synthesis, but is not blocked by mitomycin C or Colcemid. Other inducers of CSA include Mycobacterium Bacillus Calmette-Guerin, tuberculin protein preparation purified protein derivative, zymosan, and phorbol myristate. All inducing agents are specific inhibitors of the monocyte tumor cell proliferation in vitro. Latex beads, another macrophage-activating agent, are rapidly phagocytosed by PU5-1.8 cells, but neither inhibit growth nor induce CSA.