Professional antigen presenting cells in minor salivary glands in Sjogren's syndrome: Potential contribution to the histopathological diagnosis?
Professional antigen presenting cells in minor salivary glands in Sjogren's syndrome: Potential contribution to the histopathological diagnosis?
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DOI：
10.1038/labinvest.3780203
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发表时间：
2000-12-01
影响因子：
5
通讯作者：
Versnel, MA
中科院分区：
文献类型：
--
作者：
van Blokland, SCA;Wierenga-Wolf, AF;Versnel, MA
干燥综合征是一种自身免疫性疾病，其中唾液腺和泪腺中出现淋巴细胞浸润。我们已经证明，树突状细胞（DC）在淋巴细胞浸润之前会浸润非肥胖糖尿病（NOD）小鼠（一种干燥综合征的小鼠模型）的下颌下腺，这表明这些抗原呈递细胞（APC）可能在干燥综合征的发生中发挥作用。在中间阶段，DC和巨噬细胞也形成NOD唾液腺炎浸润的重要部分。为了查明 DC 和巨噬细胞是否也构成干燥综合征浸润的一部分，并确定它们是否可用于干燥综合征的组织病理学诊断，我们研究了它们在干燥综合征患者和局灶性淋巴细胞唾液腺炎 (FLS) 患者的小唾液腺 (MSG) 中的存在，但没有干燥综合征的临床或血清学标准。应用免疫组织化学，然后进行半定量分析。 DC 和巨噬细胞存在于所有味精中；然而，一方面干燥综合征和 FLS 之间的标记物表达存在明显差异，另一方面与对照组织之间的标记物表达存在明显差异。 MSG中主要观察到CD1a(+) DC和RFD9(+)巨噬细胞，其中存在局灶性淋巴细胞浸润。事实上，单个CD1a(+) DC 和RFD9(+) 巨噬细胞的弥漫性存在与MSG 中局灶性淋巴细胞浸润的存在密切相关。这表明这些细胞在味精的评估过程中可能会有所帮助。由于 APC 的检测在技术上比局灶评分更方便，因此该参数或许可以在干燥综合征的组织病理学诊断中取代局灶评分。因此，本研究促使进一步研究重点关注大量患者味精中 CD1a(+) 和 RFD9(+) 细胞的存在。
Sjogren's syndrome is an autoimmune disease in which lymphocytic infiltrates develop in the salivary and lacrimal glands. We have shown that dendritic cells (DC) infiltrate the submandibular gland of the nonobese diabetic (NOD) mouse, a mouse model for Sjogren's syndrome, before lymphocytic infiltration, suggesting that these antigen-presenting cells (APC) may play a role in the initiation of Sjogren's syndrome. In Inter stages, DC and macrophages also form an important part of the infiltrate of the NOD sialoadenitis. To find out if DC and macrophages form part of the infiltrate in Sjogren's syndrome as well, and to determine whether they may be useful in the histopathological diagnosis of Sjogren's syndrome, we studied their presence in minor salivary glands (MSG) of patients with Sjogren's syndrome and patients with focal lymphocytic sialoadenitis (FLS), but without clinical or serological criteria of Sjogren's syndrome. Immunohistochemistry was applied, followed by semiquantitative analysis. DC and macrophages were present in all MSG; however, there were clear differences in marker expression between Sjogren's syndrome and FLS, on the one hand, and control tissue, on the other hand. CD1a(+) DC and RFD9(+) macrophages were mainly observed in MSG in which a focal lymphocytic infiltrate was present. In fact, the diffuse presence of single CD1a(+) DC and RFD9(+) macrophages correlated closely with the presence of a focal lymphocytic infiltrate in the MSG. This indicates that these cells could be of help during the evaluation of a MSG. Because the detection of APC is technically less cumbersome than a focal score, this parameter may perhaps replace the focal score in the histopathological diagnosis of Sjogren's syndrome. This study therefore prompts further investigation focusing on the presence of CD1a(+) and RFD9(+) cells in the MSG of a large cohort of patients.