In vitro adhesive properties and virulence factors of Enterococcus faecalis strains
In vitro adhesive properties and virulence factors of Enterococcus faecalis strains
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DOI：
10.1016/s0923-2508(01)01291-8
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发表时间：
2002-03-01
影响因子：
2.6
通讯作者：
Joly, B
中科院分区：
文献类型：
--
作者：
Archimbaud, C;Shankar, N;Joly, B
从心内膜炎或菌血症患者或健康志愿者粪便中分离出29株粪肠球菌，研究其粘附Int-407和Girardi心脏细胞系的能力以及已知肠球菌毒力因子的存在。8株（27.6%）主要粘附于Int-407细胞。肠球菌的粘附性通过蛋白水解消化增强，表明一些细胞结合组分在用胰蛋白酶处理后变得表面暴露。肠球菌的已知潜在毒力因子依次为：肠球菌表面蛋白（72.4%）、明胶酶（58.6%）、聚集物（48.3%）和溶细胞素（17.2%）。细菌粘附与任何这些毒力因子均无显著相关性。(C)2002年，Elsevier SAS科学与医学版。All rights reserved.
Twenty-nine Enterococcus faecalis isolates from patients with endocarditis or bacteremia or from stools of healthy volunteers were investigated for their ability to adhere to Int-407 and Girardi heart cell lines and for the presence of known enterococcal virulence factors. Eight strains (27.6%) adhered predominantly to Int-407 cells. The adherence of enterococci was enhanced by proteolytic digestion, suggesting that some cell binding components become surface-exposed after treatment with trypsin. The occurrence of known potential virulence factors of enterococci among these strains was determined and was as follows: enterococcal surface protein (72.4%), gelatinase (58.6%), aggregation substance (48.3%) and cytolysin (17.2%). Bacterial adherence was not significantly associated with any of these virulence factors. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.